Anti-LDB1 antibody (ab96799)
Key features and details
- Rabbit polyclonal to LDB1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LDB1 antibody -
Description
Rabbit polyclonal to LDB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig, Zebrafish -
Immunogen
Recombinant fragment corresponding to a region within amino acids 50 and 345 of Human LDB1 (NP_003884)
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Positive control
- WB: HEK293T, A431, HeLa, HepG2 and Raji cell lysates; Mouse brain tissue lysate. IHC-P: U87 xenograft; Mouse muscle and brain tissues. IHC-Fr: Mouse brain tissue. ICC/IF: HeLa cells. IP: HEK293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
IP
Use at an assay dependent concentration. |
Target
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Function
Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state. -
Tissue specificity
Expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. -
Sequence similarities
Belongs to the LDB family. -
Domain
The dimerization domain is located in the N-terminus. -
Post-translational
modificationsUbiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8861 Human
- Entrez Gene: 16825 Mouse
- Entrez Gene: 30576 Zebrafish
- GenBank: 26002 Human
- GenBank: NM_003893 Human
- GenBank: NM_010697 Mouse
- GenBank: NM_131313 Zebrafish
- Omim: 603451 Human
see all -
Alternative names
- Carboxyl Terminal LIM Domain Binding 2 antibody
- Carboxyl-terminal LIM domain-binding protein 2 antibody
- CLIM 2 antibody
see all
Images
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HeLa cells stained for LDB1 (green) using ab96799 at 1/500 dilution in ICC/IF.
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Immunohistochemical analysis of paraffin-embedded U87 xenograft using ab96799 at a dilution of 1/100.
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Anti-LDB1 antibody (ab96799) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 47 kDa -
Immunofluorescence analysis of paraformaldheyde-fixed HeLa cells using ab96799 at a dilution of 1/200.
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All lanes : Anti-LDB1 antibody (ab96799) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa -
Anti-LDB1 antibody (ab96799) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 47 kDa -
LDB1 was immunoprecipitated from 1000 µg 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab96799. Western blot was performed from the immunoprecipitate using 2.5 μg of ab96799 at 1/1000 dilution.
Lane A: 20 μg 293T whole cell lysate/extract.
Lane B: Control with 2.5 μg of preimmune rabbit IgG.
Lane C: ab96799 IP in 293T (12% SDS-PAGE). -
Paraffin-embedded mouse muscle tissue stained for LDB1 using ab96799 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded rat middle brain tissue stained for LDB1 using ab96799 at a/500 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab96799 has been referenced in 7 publications.
- Tian Y et al. GEO Database Screening Combined with In Vitro Experiments to Study the Mechanism of hsa_circ_0003570 in Infantile Hemangiomas. Comput Math Methods Med 2022:5643742 (2022). PubMed: 35529258
- Nafria M et al. Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells. Cell Rep 31:107691 (2020). PubMed: 32460028
- Xiao D et al. Generation of self-organized sensory ganglion organoids and retinal ganglion cells from fibroblasts. Sci Adv 6:eaaz5858 (2020). PubMed: 32523990
- Ptasinska A et al. RUNX1-ETO Depletion in t(8;21) AML Leads to C/EBPa- and AP-1-Mediated Alterations in Enhancer-Promoter Interaction. Cell Rep 28:3022-3031.e7 (2019). PubMed: 31533028
- Schuetzmann D et al. Temporal autoregulation during human PU.1 locus SubTAD formation. Blood 132:2643-2655 (2018). PubMed: 30315124
- Xiao D et al. Necessity and Sufficiency of Ldb1 in the Generation, Differentiation and Maintenance of Non-photoreceptor Cell Types During Retinal Development. Front Mol Neurosci 11:271 (2018). PubMed: 30127719
- Stanulovic VS et al. LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage. Nucleic Acids Res 45:9874-9888 (2017). PubMed: 28973433