LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)

Overview

  • Product name
    LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric)
    See all Lactate dehydrogenase kits
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Quantitative
  • Sensitivity
    > 1 mU/ml
  • Range
    1 mU/ml - 100 mU/ml
  • Assay time
    0h 30m
  • Species reactivity
    Reacts with: Mammal
  • Product overview

    Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) (ab102526) quantifies LDH activity in a variety of biological samples such as serum or plasma, cells, culture medium and fermentation. In this assay LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    As LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has broad range of applications. The kit can detect 1-100 mU/ml of LDH directly in samples.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab102526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Kinetic profiles of approx 0.5 mU of a sample of pure LDH (Positive Control) and 2 μL frozen human serum using buffer as a background control.

  • NADH Standard Curve
  • Cellular necrosis was measured as LDH release after cisplatin treatment for 24 h and 48 h using ab102526. The release of LDH was apparently increased at 24 and 48 h after cisplatin treatment.

Protocols

References

This product has been referenced in:
  • Cases J  et al. Supplementation with a Polyphenol-Rich Extract, PerfLoad(®), Improves Physical Performance during High-Intensity Exercise: A Randomized, Double Blind, Crossover Trial. Nutrients 9:N/A (2017). Functional Studies ; Human . Read more (PubMed: 28441760) »
  • Wang B  et al. IL-1ß-Induced Protection of Keratinocytes against Staphylococcus aureus-Secreted Proteases Is Mediated by Human ß-Defensin 2. J Invest Dermatol 137:95-105 (2017). Functional Studies . Read more (PubMed: 27702565) »

See all 12 Publications for this product

Customer reviews and Q&As

The kit ab102526 is more suitable for measuring the LDH activity in tissue culture supernatants - It can also be used for tissue extracts.

ab65393 is only tested with cells and cell extracts - tissue extracts are yet to be tested therefore I ...

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Hello!
Based on the info you sent you basically diluted 1:10 once witht he original supernatant and then 1:10 again by using 5 ul plus assay buffer to make 50 ul total, so your final dilution is 1:100. Please let me know if you have any further que...

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The dilution is the initial sample dilution. If you have 10 µl of a sample and are diluting it 100 times and then taking in 5 µl from that diluted sample into the well, your dilution factor would be 100. In this case dilution would be 1 since you ...

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What I understand from your question is, that you would like a kit which measure lactate dehydrogenase produced from cells found in soil? In addition you would like to not detach the cells from the scaffold on which you are growing them to then re...

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Thank you for contacting us.

The two vials are actually lysophilized products. It does not say this on the datasheet, however the protocol booklet itself (which should have come with the kit and can also be downloaded from our website) gives...

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Gracias por contactarnos.

Adjunto a este mail envío el certificado de conformidad del kit ab102526.

Espero que os sea de utilidad, y en cualquier caso no dudéis en contactarnos para cualquier duda o sugerencia.

Te pido disculpas por la espera.

Siento mucho los inconvenientes causados por la confusión al etiquetar los componentes del kit. Estoy chequeando con mis compañeros responsables los lotes que tenemos actualmente en stock para co...

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Thank you for contacting us.

I am sorry that the equation provided in the protocol booklet of the Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric), ab102526, does contain a typing error. the equation should read T2-T1 as you have pointed o...

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Thanks for your enquiry regarding ab102526.

We have no specific recommendations for what kind of media you could use with this assay. I just looked it up online and it looks like phenol containing medium having ˜10% FBS will enhance the signa...

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There is a possibility that the PR in the media might interfere to some extent. To account for such interference, I would recommend using a control sample with just the plain media in replicate wells. Any signals due to any components in the media can ...

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1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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