Overview

  • Product nameAnti-LDHA antibody
    See all LDHA primary antibodies
  • Description
    Rabbit polyclonal to LDHA
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 133-310 of Human LDHA. (Uniprot:P00338)

  • Positive control
    • A431 cell lysate, 293T cell lysate, Jurkat cell lysate, Raji cell lysate and Human Lung papillory tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab125683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
  • Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 19 protein antibody
    • GSD11 antibody
    • L lactate dehydrogenase A chain antibody
    • L-lactate dehydrogenase A chain antibody
    • l7R2 antibody
    • Lactate dehydrogenase 1, A chain antibody
    • Lactate dehydrogenase A antibody
    • Lactate dehydrogenase A4 antibody
    • Lactate dehydrogenase M antibody
    • LDH A antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH muscle subunit; M LDH antibody
    • LDH-A antibody
    • LDH-M antibody
    • LDH1 antibody
    • ldha antibody
    • LDHA_HUMAN antibody
    • LDHM antibody
    • OTTMUSP00000017774 antibody
    • PIG19 antibody
    • Proliferation-inducing gene 19 antibody
    • Renal carcinoma antigen NY-REN-59 antibody
    see all

Anti-LDHA antibody images

  • Anti-LDHA antibody (ab125683) at 1/1000 dilution + A431 lysate at 30 µg

    Predicted band size : 37 kDa
  • ab125683, at 1/500 dilution, staining LDHA in Formalin-fixed, Paraffin-embedded Human Lung papillory tissue by Immunohistochemistry.
  • ab125683, at 1/500 dilution, staining LDHA in Methanol-fixed A431 cells by Immunocytochemistry/Immunofluorescence.

References for Anti-LDHA antibody (ab125683)

ab125683 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (SH-SY5Y cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification SH-SY5Y cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Nov 19 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"