Overview

  • Product name
  • Description
    Rabbit polyclonal to LDHA
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Chimpanzee, Spider monkey
  • Immunogen

    Synthetic peptide designed within residues: ATLKDQLIYN LLKEEQTPQN KITVVGVGAV GMACAISILM KDLADELALV, corresponding to N terminal amino acids 2-51 of Human LDHA (NP_005557)

  • Positive control
    • Human placenta lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Pathway
    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease
    Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
  • Sequence similarities
    Belongs to the LDH/MDH superfamily. LDH family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 19 protein antibody
    • GSD11 antibody
    • L lactate dehydrogenase A chain antibody
    • L-lactate dehydrogenase A chain antibody
    • l7R2 antibody
    • Lactate dehydrogenase 1, A chain antibody
    • Lactate dehydrogenase A antibody
    • Lactate dehydrogenase A4 antibody
    • Lactate dehydrogenase M antibody
    • LDH A antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH muscle subunit; M LDH antibody
    • LDH-A antibody
    • LDH-M antibody
    • LDH1 antibody
    • ldha antibody
    • LDHA_HUMAN antibody
    • LDHM antibody
    • OTTMUSP00000017774 antibody
    • PIG19 antibody
    • Proliferation-inducing gene 19 antibody
    • Renal carcinoma antigen NY-REN-59 antibody
    see all

Images

  • Immunohistochemistry (Formlin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling LDHA with ab84716.

  • Anti-LDHA antibody (ab84716) at 1 µg/ml + Human placenta lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 37 kDa

References

This product has been referenced in:
  • Cui XG  et al. HIF1/2a mediates hypoxia-induced LDHA expression in human pancreatic cancer cells. Oncotarget 8:24840-24852 (2017). WB ; Human . Read more (PubMed: 28193910) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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