The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:1562500.
RelevanceLebercilin encodes a protein that is thought to be involved in centrosomal or ciliary functions. Mutations in this gene cause Leber congenital amaurosis type V. Alternative splicing results in two transcript variants.
Cellular localizationCytoplasm, cytoskeleton. Cell projection, cilium axoneme. Cell projection, cilium basal body. Note: In non ciliated cells, localizes to the centrosome and its associated microtubule array.