The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
In a sandwich ELISA (assuming 100µl/well), a concentration of 1-2 µg/ml of this antibody will detect at least 0.03ng/well of recombinant human LEC
FunctionShows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.
Tissue specificityMainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.