Anti-LECT2 antibody [OTI2A11] (ab119429)
Key features and details
- Mouse monoclonal [OTI2A11] to LECT2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-LECT2 antibody [OTI2A11]
See all LECT2 primary antibodies -
Description
Mouse monoclonal [OTI2A11] to LECT2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human LECT2 aa 1-200. Produced in E.coli (NP_002293). aa 1-18 are the signal peptide, immunogen is to the full length mature chain.
Database link: O14960 -
Positive control
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY LECT2 cDNA. IHC-P: Human breast, Kidney, Lung carcinoma, Ovary adenocarcinoma, Pancreas, Endometrium adenocarcinoma and Tonsil tissues.
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General notes
The clone number has been updated from 2A11 to OTI2A11, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI2A11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Predicted molecular weight: 16 kDa.
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IHC-P |
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500. Predicted molecular weight: 16 kDa. |
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation. -
Tissue specificity
Highly expressed in adult and fetal liver and weakly in testis. Not expressed in bone marrow. -
Sequence similarities
Belongs to the LECT2/MIM-1 family. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3950 Human
- Omim: 602882 Human
- SwissProt: O14960 Human
- Unigene: 512580 Human
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Alternative names
- chm II antibody
- chm2 antibody
- Chondromodulin II antibody
see all
Images
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All lanes : Anti-LECT2 antibody [OTI2A11] (ab119429) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY LECT2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 16 kDa -
Paraffin-embedded human breast tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human lung carcinoma tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human ovary adenocarcinoma tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreas tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium adenocarcinoma tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human tonsil tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab119429 has been referenced in 2 publications.
- Shirasaki T et al. Leukocyte cell-derived chemotaxin 2 is an antiviral regulator acting through the proto-oncogene MET. Nat Commun 13:3176 (2022). PubMed: 35676290
- Xu M et al. LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis. Cell 178:1478-1492.e20 (2019). PubMed: 31474362