Anti-LEF1 antibody (ab124271)

Overview

• Product name
  Anti-LEF1 antibody
  See all LEF1 primary antibodies
• Description
  Rabbit polyclonal to LEF1
• Tested applications
  Suitable for: WB, IPmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
• Immunogen

  Synthetic peptide, corresponding to a region within amino acids 250-300 of Human LEF1 (NP_057353.1).

• Positive control
  • Purchase matching WB positive control:Recombinant Human LEF1 protein
  • Jurkat whole cell lysate

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer
  Preservative: 0.09% Sodium azide
  Constituent: 99% Tris citrate/phosphate
  Note: pH 7 to 8
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Purification notes
  ab124271 was affinity purified using an epitope specific to LEF1 immobilized on solid support .
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HMG Box

  • Stem Cells
  • Lineage Markers
  • Mesoderm

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Nuclear

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • PTC & Wnt pathway

  • Developmental Biology
  • Lineage specification
  • Mesoderm

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Positive Controls
  • Jurkat whole cell lysate (ab30128)
  • Jurkat whole cell lysate (ab7899)

Applications

Target

• Function
  Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
• Tissue specificity
  Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
• Sequence similarities
  Belongs to the TCF/LEF family.
  Contains 1 HMG box DNA-binding domain.
• Domain
  Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
• Cellular localization
  Nucleus. Found in nuclear bodies upon PIASG binding.

• Target information above from: UniProt accession Q9UJU2 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 51176 Human
  • Entrez Gene: 16842 Mouse
  • Entrez Gene: 161452 Rat
  • Omim: 153245 Human
  • SwissProt: Q9UJU2 Human
  • SwissProt: P27782 Mouse
  • SwissProt: Q9QXN1 Rat
  • Unigene: 726506 Human

  • Unigene: 255219 Mouse
  • Unigene: 21926 Rat

  see all
• Alternative names
  • DKFZp586H0919 antibody
  • FLJ46390 antibody
  • LEF 1 antibody

  • LEF-1 antibody
  • Lef1 antibody
  • LEF1_HUMAN antibody
  • Lymphoid enhancer binding factor 1 antibody
  • Lymphoid enhancer-binding factor 1 antibody
  • T cell specific transcription factor 1 alpha antibody
  • T cell-specific transcription factor 1-alpha antibody
  • TCF 1 alpha antibody
  • TCF1 alpha antibody
  • TCF1-alpha antibody
  • TCF10 antibody
  • TCF1alpha antibody
  • TCF7L3 antibody
  • Transcription factor T cell specific 1 alpha antibody

  see all

Protocols

• Western blot protocols
• Immunoprecipitation protocols