Overview

  • Product name
  • Description
    Rabbit polyclonal to LEF1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LEF1 aa 350-399 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    ELARKERQLHM QLYPGWSARD NYGKKKKRKR EKLQESASGT GPRMTAAYI


    Database link: Q9UJU2

  • Positive control
    • Mouse lymph node and human colon carcinoma lysates; rat liver tissue and human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab217378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
  • Tissue specificity
    Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
  • Sequence similarities
    Belongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • Domain
    Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
  • Cellular localization
    Nucleus. Found in nuclear bodies upon PIASG binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586H0919 antibody
    • FLJ46390 antibody
    • LEF 1 antibody
    • LEF-1 antibody
    • Lef1 antibody
    • LEF1_HUMAN antibody
    • Lymphoid enhancer binding factor 1 antibody
    • Lymphoid enhancer-binding factor 1 antibody
    • T cell specific transcription factor 1 alpha antibody
    • T cell-specific transcription factor 1-alpha antibody
    • TCF 1 alpha antibody
    • TCF1 alpha antibody
    • TCF1-alpha antibody
    • TCF10 antibody
    • TCF1alpha antibody
    • TCF7L3 antibody
    • Transcription factor T cell specific 1 alpha antibody
    see all

Anti-LEF1 antibody images

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat liver tissue labeling LEF1 with ab217378 at 1/200 dilution followed by secondary detection and DAB staining.

  • All lanes : Anti-LEF1 antibody (ab217378) at 1/200 dilution

    Lane 1 : rat brain lysate
    Lane 2 : human colon carcinoma lysate

    Secondary
    Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

    Predicted band size : 44 kDa
  • Anti-LEF1 antibody (ab217378) at 1/300 dilution + Mouse lymph node lysate

    Secondary
    Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/500 dilution

    Predicted band size : 44 kDa
  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded human colon carcinoma tissue labeling LEF1 with ab217378 at 1/200 dilution followed by secondary detection and DAB staining.

References for Anti-LEF1 antibody (ab217378)

ab217378 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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