The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 44 kDa).
FunctionParticipates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Tissue specificityDetected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
Sequence similaritiesBelongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain.
DomainProline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
Cellular localizationNucleus. Found in nuclear bodies upon PIASG binding.
T cell specific transcription factor 1 alpha antibody
T cell-specific transcription factor 1-alpha antibody
TCF 1 alpha antibody
TCF1 alpha antibody
Transcription factor T cell specific 1 alpha antibody
Anti-LEF1 antibody images
Western blot - LEF1 antibody (ab28316)
All lanes : Anti-LEF1 antibody (ab28316) at 5 µg/ml
Lane 1 : Mock Lane 2 : TCF1 Lane 3 : TCF4 Lane 4 : Jurkat cell extract Lane 5 : Full length LEF1 Lane 6 : Full length LEF1 Ø HMG Lane 7 : Recombinant full length LEF1 at 0.01 µg Lane 8 : LEF1 amino acids 1 - 192 Lane 9 : LEF1 amino acids 1 - 276
Predicted band size : 44 kDa
References for Anti-LEF1 antibody (ab28316)
has not yet been referenced specifically in any publications.
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