Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab52017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa). The observed molecular weight corresponds to earlier findings in literature (Yasumoto et al , EMBO J. 2002 Jun 3;21(11):2703-14.; PMID: 12032083). Immunofluorescence: This antibody was successfully used to stain LEF1 in Zebrafish fish fins. Fins were fixed with paraformaldehyde at 4°C (See PMID 19015519).
EMSA Use at an assay dependent concentration. PubMed: 22011226

Target

  • FunctionParticipates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
  • Tissue specificityDetected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
  • Sequence similaritiesBelongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • DomainProline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
  • Cellular localizationNucleus. Found in nuclear bodies upon PIASG binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586H0919 antibody
    • FLJ46390 antibody
    • LEF 1 antibody
    • LEF-1 antibody
    • Lef1 antibody
    • LEF1_HUMAN antibody
    • Lymphoid enhancer binding factor 1 antibody
    • Lymphoid enhancer-binding factor 1 antibody
    • T cell specific transcription factor 1 alpha antibody
    • T cell-specific transcription factor 1-alpha antibody
    • TCF 1 alpha antibody
    • TCF1 alpha antibody
    • TCF1-alpha antibody
    • TCF10 antibody
    • TCF1alpha antibody
    • TCF7L3 antibody
    • Transcription factor T cell specific 1 alpha antibody
    see all

Anti-LEF1 antibody images

References for Anti-LEF1 antibody (ab52017)

This product has been referenced in:
  • Chatterjee S  et al. Conserved and non-conserved enhancers direct tissue specific transcription in ancient germ layer specific developmental control genes. BMC Dev Biol 11:63 (2011). EMSA ; Zebrafish . Read more (PubMed: 22011226) »
  • Thatcher EJ  et al. Regulation of zebrafish fin regeneration by microRNAs. Proc Natl Acad Sci U S A 105:18384-9 (2008). Zebrafish . Read more (PubMed: 19015519) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam. I believe that both choices that you have made are excellent. Both are covered by our Abpromise guarantee so you need not be concerned that the product will work for you should you use them in the manner consistant with ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"