Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab85052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    Peptide ELISA: 1/40000 - 1/80000.
    WB: 1/200 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 44 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
    • Tissue specificity
      Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
    • Sequence similarities
      Belongs to the TCF/LEF family.
      Contains 1 HMG box DNA-binding domain.
    • Domain
      Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
    • Cellular localization
      Nucleus. Found in nuclear bodies upon PIASG binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp586H0919 antibody
      • FLJ46390 antibody
      • LEF 1 antibody
      • LEF-1 antibody
      • Lef1 antibody
      • LEF1_HUMAN antibody
      • Lymphoid enhancer binding factor 1 antibody
      • Lymphoid enhancer-binding factor 1 antibody
      • T cell specific transcription factor 1 alpha antibody
      • T cell-specific transcription factor 1-alpha antibody
      • TCF 1 alpha antibody
      • TCF1 alpha antibody
      • TCF1-alpha antibody
      • TCF10 antibody
      • TCF1alpha antibody
      • TCF7L3 antibody
      • Transcription factor T cell specific 1 alpha antibody
      see all

    Anti-LEF1 antibody images

    • ICC/IF image of ab85052 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85052 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-LEF1 antibody (ab85052) at 1/500 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat cell lysate


      Predicted band size : 44 kDa
      Observed band size : 44 kDa

    References for Anti-LEF1 antibody (ab85052)

    This product has been referenced in:
    • Jiang S  et al. Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway. Mol Med Rep 14:1891-900 (2016). WB ; Human . Read more (PubMed: 27432616) »

    See 1 Publication for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brain tissue (hippocampus))
    Permeabilization
    Yes - 0.05% triton
    Specification
    Brain tissue (hippocampus)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 01 2016

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (PC12 (from rat) Adult Hipocampal Neural Stem Cells)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    60 µg
    Specification
    PC12 (from rat) Adult Hipocampal Neural Stem Cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Mr. Tomás Armenteros

    Verified customer

    Submitted May 06 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Embryonic stem cells)
    Permeabilization
    Yes - Triton X-100 (0.5%)
    Specification
    Embryonic stem cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5%
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 01 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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