Anti-LEF1 antibody [EP2030Y] (ab53293)


  • Product nameAnti-LEF1 antibody [EP2030Y]
    See all LEF1 primary antibodies
  • Description
    Rabbit monoclonal [EP2030Y] to LEF1
  • Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in human LEF1.

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.



Our Abpromise guarantee covers the use of ab53293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 44 kDa).
Flow Cyt 1/20. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionParticipates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
    • Tissue specificityDetected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
    • Sequence similaritiesBelongs to the TCF/LEF family.
      Contains 1 HMG box DNA-binding domain.
    • DomainProline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
    • Cellular localizationNucleus. Found in nuclear bodies upon PIASG binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp586H0919 antibody
      • FLJ46390 antibody
      • LEF 1 antibody
      • LEF-1 antibody
      • Lef1 antibody
      • LEF1_HUMAN antibody
      • Lymphoid enhancer binding factor 1 antibody
      • Lymphoid enhancer-binding factor 1 antibody
      • T cell specific transcription factor 1 alpha antibody
      • T cell-specific transcription factor 1-alpha antibody
      • TCF 1 alpha antibody
      • TCF1 alpha antibody
      • TCF1-alpha antibody
      • TCF10 antibody
      • TCF1alpha antibody
      • TCF7L3 antibody
      • Transcription factor T cell specific 1 alpha antibody
      see all

    Anti-LEF1 antibody [EP2030Y] images

    • ab53293 staining LEF1 in RAMOS (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain and the negative control was PBS only.

    • Anti-LEF1 antibody [EP2030Y] (ab53293) at 1/1000 dilution + Jurkat whole cell lysate at 10 µg

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 44 kDa
      Observed band size : 25-57 kDa (why is the actual band size different from the predicted?)

      Exposure time : 1 minute

      Blocking/Diluting buffer 5% NFDM/TBST

    • Ab53293 (1:50) staining human LEF1 in human colon adenocarcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Flow cytometric analysis of permeabilized Jurkat cells using ab53293 (red) or a rabbit IgG (negative) (green).

    References for Anti-LEF1 antibody [EP2030Y] (ab53293)

    This product has been referenced in:
    • Zhang X  et al. MicroRNA-195 suppresses colorectal cancer cells proliferation via targeting FGF2 and regulating Wnt/ß-catenin pathway. Am J Cancer Res 6:2631-2640 (2016). WB ; Human . Read more (PubMed: 27904776) »
    • Shaikh LH  et al. LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal. J Clin Endocrinol Metab 100:E836-44 (2015). IHC ; Human . Read more (PubMed: 25915569) »

    See all 6 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Pig Tissue sections (unerupted tooth buds)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citric Acid monobasic pH6
    Permeabilization No
    Specification unerupted tooth buds
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative 10% Formalin

    Abcam user community

    Verified customer

    Submitted Apr 27 2017

    Thank you for your inquiry.
    The exact immunogen sequence is proprietary information and I unfortunately do not have access to it.
    But the lab confirmed that all isoforms should be recognized by ab53293 since all isoforms contain the immunogen...

    Read More

    Thank you for taking the time to contact us. I am sorry to hearthe customer has some concerns regarding the results from this antibody.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and...

    Read More

    Thank you for contacting Abcam.

    I am sorry that you are having problems with ab53293 in western blot. If you are looking for a positive control then I would recommend ab7899:

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (REH B cell)
    Specification REH B cell
    Fixation Paraformaldehyde
    Permeabilization Yes - saponin

    Abcam user community

    Verified customer

    Submitted Apr 01 2010