Recombinant
RabMAb

Anti-LEF1 antibody [EP2030Y] (ab53293)

Overview

  • Product name
    Anti-LEF1 antibody [EP2030Y]
    See all LEF1 primary antibodies
  • Description
    Rabbit monoclonal [EP2030Y] to LEF1
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in human LEF1.

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab53293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 44 kDa).
Flow Cyt 1/20. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
    • Tissue specificity
      Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
    • Sequence similarities
      Belongs to the TCF/LEF family.
      Contains 1 HMG box DNA-binding domain.
    • Domain
      Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
    • Cellular localization
      Nucleus. Found in nuclear bodies upon PIASG binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp586H0919 antibody
      • FLJ46390 antibody
      • LEF 1 antibody
      • LEF-1 antibody
      • Lef1 antibody
      • LEF1_HUMAN antibody
      • Lymphoid enhancer binding factor 1 antibody
      • Lymphoid enhancer-binding factor 1 antibody
      • T cell specific transcription factor 1 alpha antibody
      • T cell-specific transcription factor 1-alpha antibody
      • TCF 1 alpha antibody
      • TCF1 alpha antibody
      • TCF1-alpha antibody
      • TCF10 antibody
      • TCF1alpha antibody
      • TCF7L3 antibody
      • Transcription factor T cell specific 1 alpha antibody
      see all

    Anti-LEF1 antibody [EP2030Y] images

    • ab53293 staining LEF1 in RAMOS (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain and the negative control was PBS only.

    • Anti-LEF1 antibody [EP2030Y] (ab53293) at 1/1000 dilution + Jurkat whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 44 kDa
      Observed band size : 25-57 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Blocking/Diluting buffer 5% NFDM/TBST

    • Ab53293 (1:50) staining human LEF1 in human colon adenocarcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Flow cytometric analysis of permeabilized Jurkat cells using ab53293 (red) or a rabbit IgG (negative) (green).

    References for Anti-LEF1 antibody [EP2030Y] (ab53293)

    This product has been referenced in:
    • Zhang X  et al. MicroRNA-195 suppresses colorectal cancer cells proliferation via targeting FGF2 and regulating Wnt/ß-catenin pathway. Am J Cancer Res 6:2631-2640 (2016). WB ; Human . Read more (PubMed: 27904776) »
    • Shaikh LH  et al. LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal. J Clin Endocrinol Metab 100:E836-44 (2015). IHC ; Human . Read more (PubMed: 25915569) »

    See all 6 Publications for this product

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    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (unerupted tooth buds)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM citric Acid monobasic pH6
    Permeabilization
    No
    Specification
    unerupted tooth buds
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    10% Formalin
    Username

    Abcam user community

    Verified customer

    Submitted Apr 27 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (REH B cell)
    Specification
    REH B cell
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - saponin
    Username

    Abcam user community

    Verified customer

    Submitted Apr 01 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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