Overview

  • Product nameAnti-LEF1 (phospho S42) antibody
    See all LEF1 primary antibodies
  • Description
    Rabbit polyclonal to LEF1 (phospho S42)
  • Specificityab74067 detects endogenous levels of LEF1 only when phosphorylated at serine 42.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human LEF1 around the phosphorylation site of serine 42 (E-I-SP-H-P).

  • Positive control
    • 293 cell extracts treated with paclitaxel (1uM, 24hours).

Properties

Applications

Our Abpromise guarantee covers the use of ab74067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA 1/20000.

Target

  • FunctionParticipates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
  • Tissue specificityDetected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
  • Sequence similaritiesBelongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • DomainProline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
  • Cellular localizationNucleus. Found in nuclear bodies upon PIASG binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586H0919 antibody
    • FLJ46390 antibody
    • LEF 1 antibody
    • LEF-1 antibody
    • Lef1 antibody
    • LEF1_HUMAN antibody
    • Lymphoid enhancer binding factor 1 antibody
    • Lymphoid enhancer-binding factor 1 antibody
    • T cell specific transcription factor 1 alpha antibody
    • T cell-specific transcription factor 1-alpha antibody
    • TCF 1 alpha antibody
    • TCF1 alpha antibody
    • TCF1-alpha antibody
    • TCF10 antibody
    • TCF1alpha antibody
    • TCF7L3 antibody
    • Transcription factor T cell specific 1 alpha antibody
    see all

Anti-LEF1 (phospho S42) antibody images

  • All lanes : Anti-LEF1 (phospho S42) antibody (ab74067) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with paclitaxel (1uM, 24hours)
    Lane 2 : 293 cell extracts treated with paclitaxel (1uM, 24hours) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 44 kDa
    Observed band size : 44 kDa

References for Anti-LEF1 (phospho S42) antibody (ab74067)

ab74067 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab74067.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"