Overview

  • Product name
  • Description
    Rabbit polyclonal to Lefty
  • Specificity
    This antibody has been tested against recombinant protein only (human LeftyA and mouse Lefty1). I is expected to cross react with Human LeftyB as there is 100% homology of the immunogen with Human LeftyB.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Lefty.

    (Peptide available as ab25701.)

  • Positive control
    • recombinant Lefty1/ LeftyA

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 1 µg/ml. Detects a bands of approximately 44, 38 and 34 kDa (predicted molecular weight: 41 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      During vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • AI450052 antibody
      • Ebaf antibody
      • Left right determination factor 1 antibody
      • Left right determination factor antibody
      • Left right determination factor B antibody
      • left-right determination factor antibody
      • LEFT-RIGHT DETERMINATION FACTOR 1; LEFTY1 antibody
      • left-right determination, factor B antibody
      • LEFT-RIGHT DETERMINATION, FACTOR B; LEFTY B antibody
      • LEFTB antibody
      • LEFTY 1 antibody
      • Lefty antibody
      • lefty-1 antibody
      • LEFTY1 antibody
      • LEFTY1, MOUSE, HOMOLOG OF antibody
      • LEFTYB antibody
      • OTTHUMP00000035572 antibody
      • Protein lefty-1 antibody
      • Protein lefty-B antibody
      • RGD1561867 antibody
      • Stimulated by retinoic acid gene 3 protein antibody
      • STRA3 antibody
      • TGF beta 4 antibody
      • TGF-beta-4 antibody
      • Tgfb4 antibody
      • Transforming growth factor beta 4 antibody
      • Transforming growth factor beta-4 antibody
      see all

    Images

    • ICC/IF image of ab22569 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22569, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
    • All lanes : Anti-Lefty antibody (ab22569) at 1 µg/ml

      Lane 1 : Mouse Lefty1 Recombinant Protein
      Lane 2 : Human LeftyA Recombinant Protein

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

      Predicted band size : 41 kDa
      Observed band size : 34,38,44 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:

    See all 3 Publications for this product

    Customer reviews and Q&As

    Vielen Dank für Ihr Interesse an unseren Produkten.


    Wir haben diese beiden Antikörper leider noch gar nicht an endogenen Proben getestet, so dass wir leider keine Aussage treffen können, welcher besser mit Mausproben funkt...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (E6.5 embryo in decidua)
    Specification
    E6.5 embryo in decidua
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric Acid
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Username

    Dr. Jesse Mager

    Verified customer

    Submitted Feb 13 2009

    Thank you for your enquiry regarding ab22569. This antibody will recognise human lefty1 and left2 according to a BLAST search with the immunogen sequence though it has only been tested against recombinant Lefty1. Regarding its cross reactivity to mou...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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