Anti-LEFTY2 antibody [7C5G1H6H10] (ab115224)

Overview

  • Product name
    Anti-LEFTY2 antibody [7C5G1H6H10]
    See all LEFTY2 primary antibodies
  • Description
    Mouse monoclonal [7C5G1H6H10] to LEFTY2
  • Specificity
    May cross react with the highly homologous LEFTY1.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A recombinant form of 6X HIS tagged LEFTY2 (Human)

  • Positive control
    • Human uterus, myometrium tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 41 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.
  • Tissue specificity
    Mesenchymal cells of the endometrial stroma.
  • Involvement in disease
    Defects in LEFTY2 are the cause of left-right axis malformations (LRAM) [MIM:601877]. The defect includes left pulmonary isomerism, with cardiac anomalies characterized by complete atrioventricular canal defect and hypoplastic left ventricle, and interrupted inferior vena cava.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Developmental stage
    Transiently expressed before and during menstrual bleeding.
  • Post-translational
    modifications
    The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • EBAF antibody
    • Endometrial bleeding associated factor (left right determination factor A transforming growth factor beta superfamily) antibody
    • Endometrial bleeding associated factor antibody
    • Endometrial bleeding-associated factor antibody
    • Left right determination factor 2 antibody
    • Left right determination factor A antibody
    • Left-right determination factor 2 antibody
    • Left-right determination factor A antibody
    • LEFTA antibody
    • LEFTY 2 antibody
    • LEFTY2 antibody
    • LEFTYA antibody
    • LFTY2 transforming growth factor, beta -4 antibody
    • LFTY2, mouse, homolog of antibody
    • LFTY2_HUMAN antibody
    • MGC46222 antibody
    • Protein lefty 2 antibody
    • Protein lefty A antibody
    • Protein lefty-2 antibody
    • Protein lefty-A antibody
    • Protein lefty2 antibody
    • PSEC0024 antibody
    • TGF beta 4 antibody
    • TGF-beta-4 antibody
    • TGFB4 antibody
    • Transforming growth factor beta 4 antibody
    • Transforming growth factor beta-4 antibody
    see all

Images

  • Human uterus, myometrium (formalin fixed, paraffin embedded) stained with ab115224 at 10 ug/ml followed by biotinylated anti Mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.
  • Anti-LEFTY2 antibody [7C5G1H6H10] (ab115224) at 1/2000 dilution + partially purified recombinant 6X His tagged human LEFTY2 at 1000 µg

    Predicted band size : 41 kDa

References

ab115224 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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