The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 41 kDa.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.
Mesenchymal cells of the endometrial stroma.
Involvement in disease
Defects in LEFTY2 are the cause of left-right axis malformations (LRAM) [MIM:601877]. The defect includes left pulmonary isomerism, with cardiac anomalies characterized by complete atrioventricular canal defect and hypoplastic left ventricle, and interrupted inferior vena cava.
Belongs to the TGF-beta family.
Transiently expressed before and during menstrual bleeding.
The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.
Human uterus, myometrium (formalin fixed, paraffin embedded) stained with ab115224 at 10 ug/ml followed by biotinylated anti Mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.
Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (ab115224)
Anti-LEFTY2 antibody [7C5G1H6H10] (ab115224) at 1/2000 dilution + partially purified recombinant 6X His tagged human LEFTY2 at 1000 µg