Anti-Legionella pneumophila antibody (Biotin) (ab20527)

Overview

  • Product name
    Anti-Legionella pneumophila antibody (Biotin)
    See all Legionella pneumophila primary antibodies
  • Description
    Rabbit polyclonal to Legionella pneumophila (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Reacts with Legionella pneumophila serogroups 1-12 in IFA. Recognises all antigens of intact microorganism. Antiserum is not absorbed and may cross-react with related microorganisms.
  • Tested applications
    Suitable for: ICC/IF, ELISAmore details
  • Species reactivity
    Reacts Legionella pneumophila serogroups 1-12, other organisms not tested.
  • Immunogen

    A whole cell preparation of Legionella pneumophila; ATCC #33152

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IF: Acetone-fixed organisms. 1/800.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Legionella pneumophila is a flagellated gram negative bacterium found primarily in warm water environments. Legionella pneumophila causes a type of pneumonia called Legionnaire disease and a milder condition called Pontiac fever. Infection is acquired through inhalation of the microorganism in water mists (e.g. from air conditioning, cooling towers, whirlpool spas and showers mists). A number of risk factors for acquiring Legionnaire disease have been identified, including age, smoking, chronic lung disease, cancer, and immunosuppression.

    References for Anti-Legionella pneumophila antibody (Biotin) (ab20527)

    ab20527 has not yet been referenced specifically in any publications.

    Product Wall

    ab20561 and ab20527 are produced in the same lab and in exactly the same way, using a whole cell preparation of Legionella pneumophila; ATCC #33152 to immunise the rabbits. They are therefore the same antibody but conjugated to different molecules. ab2...

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    Thank you for contacting us and for presenting your questions so clearly! To amplify the the signal from the FITC-conjugated anti-Legionella antibody, your idea of using an Alexa Fluor 488-conjugated anti-FITC is a good one. We unfortunately do not...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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