The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
Use a concentration of 2 - 5 µg/ml.
Use a concentration of 0.01 - 0.1 µg/ml.
Use a concentration of 0.5 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionHas a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
Tissue specificityUbiquitous. Particularly abundant in kidney, heart and placenta.
Sequence similaritiesBelongs to the peptidase C13 family.