For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit monoclonal [EPR14718] to Legumain
Mouse, Rat, Human
Recombinant fragment within Human Legumain aa 200-350. The exact sequence is proprietary.
Database link: Q99538
Human fetal kidney lysate; JAR, HeLa and 293 cell lysates.
This product is a recombinant rabbit monoclonal antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
Ubiquitous. Particularly abundant in kidney, heart and placenta.
Belongs to the peptidase C13 family.
Information by UniProt
Asparaginyl endopeptidase antibody
cysteine 1 antibody
Western blot - Anti-Legumain antibody [EPR14718] (ab183028)
All lanes :
Anti-Legumain antibody [EPR14718] (ab183028) at 1/10000 dilution
Lane 1 :
Human fetal kidney lysate
Lane 2 :
JAR cell ysate
Lane 3 :
HeLa cell lysate
Lane 4 :
293 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size:
Actual band at: 36kDa, 46kDa, 56kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"