Recombinant
RabMAb

Anti-Legumain antibody [EPR14718] (ab183028)

Overview

  • Product name
    Anti-Legumain antibody [EPR14718]
    See all Legumain primary antibodies
  • Description
    Rabbit monoclonal [EPR14718] to Legumain
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Legumain aa 200-350. The exact sequence is proprietary.
    Database link: Q99538

  • Positive control
    • Human fetal kidney lysate; JAR, HeLa and 293 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab183028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 49 kDa.

Target

  • Function
    Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
  • Tissue specificity
    Ubiquitous. Particularly abundant in kidney, heart and placenta.
  • Sequence similarities
    Belongs to the peptidase C13 family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • AEP antibody
    • Asparaginyl endopeptidase antibody
    • cysteine 1 antibody
    • Cysteine protease 1 antibody
    • EC 3.4.22.34 antibody
    • Legumain antibody
    • LGMN antibody
    • LGMN_HUMAN antibody
    • LGMN1 antibody
    • Protease antibody
    • Protease cysteine 1 (legumain) antibody
    • Protease cysteine 1 antibody
    • PRSC1 antibody
    see all

Anti-Legumain antibody [EPR14718] images

  • All lanes : Anti-Legumain antibody [EPR14718] (ab183028) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : JAR cell ysate
    Lane 3 : HeLa cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 49 kDa


    Exposure time : 3 minutes

    Actual band at: 36kDa, 46kDa, 56kDa

     

References for Anti-Legumain antibody [EPR14718] (ab183028)

ab183028 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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