The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
FunctionThe PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. May be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.
Tissue specificityHighly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver.
Immunocytochemistry/ Immunofluorescence - LEO1 antibody (ab33157)This image is courtesy of an anonymous Abreview.
ab33157 staining LEO1 in Mouse ES cells by Immunocytochemistry/ Immunofluorescence. The cells were methanol fixed and blocked in 0.2% Fish scale gelatin for 1 hour at 25°C. Primary antibody was used at 1/100 dilution (0.2% Fish scale gelatin in PBS) and incubated with sample for 20 minutes at 25°C. An Alexa Fluor® 488 conjugated Donkey polyclonal to rabbit IgG was used as secondary at 1/500 dilution. Image shows green color staining with ab33157 and blue with DAPI.