Overview

  • Product nameAnti-LEO1 antibody
    See all LEO1 primary antibodies
  • Description
    Mouse polyclonal to LEO1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide: SDDEKWGRED KSDQSDDEKI QNSDDEERAQ GSDEDKLQNS DDDEKMQNTD DEERPQLSDD ERQQLSEEEK ANSDDERPVA SDNDDEKQNS DDEEQPQLSD , corresponding to amino acids 140-240 of Human LEO1

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 75 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionThe PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. May be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.
  • Tissue specificityHighly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver.
  • Sequence similaritiesBelongs to the LEO1 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gm185 antibody
    • LEO1 antibody
    • LEO1_HUMAN antibody
    • LOC123439 antibody
    • Paf1/RNA polymerase II complex component antibody
    • Replicative senescence down regulated leo1 like protein antibody
    • Replicative senescence down-regulated leo1-like protein antibody
    • Replicative senescence downregulated leo1 like antibody
    • RNA polymerase associated protein LEO1 antibody
    • RNA polymerase-associated protein LEO1 antibody
    • Senescence downregulated leo1 like antibody
    see all

Anti-LEO1 antibody images

  • All lanes : Anti-LEO1 antibody (ab43770) at 1/1000 dilution

    Lane 1 : Total protein extract from E coli with ~50ng to 100ng of GST fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) conjugated to Horseradish peroxidase at 1/5000 dilution

    Predicted band size : 75 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-LEO1 antibody (ab43770)

This product has been referenced in:
  • Auclair S  et al. Absence of cumulus cells during in vitro maturation affects lipid metabolism in bovine oocytes. Am J Physiol Endocrinol Metab 304:E599-613 (2013). Read more (PubMed: 23321473) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"