Overview

  • Product nameAnti-LEO1 antibody - N-terminal
    See all LEO1 primary antibodies
  • Description
    Rabbit polyclonal to LEO1 - N-terminal
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the N-terminal region of human LEO1.

  • Positive control
    • HL60 cell line lysate. 293 cell lysate transiently transfected with the LEO1 gene.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab75721 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250. Predicted molecular weight: 75 kDa.
ELISA 1/1000.

Target

  • FunctionThe PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. May be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.
  • Tissue specificityHighly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver.
  • Sequence similaritiesBelongs to the LEO1 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gm185 antibody
    • LEO1 antibody
    • LEO1_HUMAN antibody
    • LOC123439 antibody
    • Paf1/RNA polymerase II complex component antibody
    • Replicative senescence down regulated leo1 like protein antibody
    • Replicative senescence down-regulated leo1-like protein antibody
    • Replicative senescence downregulated leo1 like antibody
    • RNA polymerase associated protein LEO1 antibody
    • RNA polymerase-associated protein LEO1 antibody
    • Senescence downregulated leo1 like antibody
    see all

Anti-LEO1 antibody - N-terminal images

  • Anti-LEO1 antibody - N-terminal (ab75721) at 1/50 dilution + HL60 cell line lysate at 35 µg

    Predicted band size : 75 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-LEO1 antibody - N-terminal (ab75721) at 1/100 dilution

    Lane 1 : Nontransfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the LEO1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 75 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

References for Anti-LEO1 antibody - N-terminal (ab75721)

ab75721 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"