Recombinant
RabMAb

Anti-LEPRE1 antibody [EPR10193(B)] (ab154799)

Overview

  • Product name
    Anti-LEPRE1 antibody [EPR10193(B)]
    See all LEPRE1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10193(B)] to LEPRE1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LEPRE1. The exact sequence is proprietary.

  • Positive control
    • HeLa, HepG2, A549 and Human placenta lysates; HepG2 and A549 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts.
    • Involvement in disease
      Defects in LEPRE1 are the cause of osteogenesis imperfecta type 8 (OI8) [MIM:610915]. A connective tissue disorder characterized by disproportionate short stature, severe osteoporosis, shortening of the long bones, white sclerae, a round face and a short barrel-shaped chest.
    • Sequence similarities
      Belongs to the leprecan family.
      Contains 1 Fe2OG dioxygenase domain.
      Contains 4 TPR repeats.
    • Post-translational
      modifications
      O-glycosylated; chondroitin sulfate.
    • Cellular localization
      Endoplasmic reticulum. Secreted > extracellular space > extracellular matrix. Secreted into the extracellular matrix as a chondroitin sulfate proteoglycan.
    • Information by UniProt
    • Database links
    • Alternative names
      • GROS 1 antibody
      • GROS1 antibody
      • Growth suppressor 1 antibody
      • LEPRE 1 antibody
      • Lepre1 antibody
      • Leprecan 1 antibody
      • Leprecan antibody
      • Leprecan-1 antibody
      • Leprecan1 antibody
      • Leucine proline enriched proteoglycan (leprecan) 1 antibody
      • Leucine proline enriched proteoglycan 1 antibody
      • Leucine- and proline-enriched proteoglycan 1 antibody
      • MGC117314 antibody
      • OI8 antibody
      • P3H1 antibody
      • P3H1_HUMAN antibody
      • Prolyl 3 hydroxylase 1 antibody
      • Prolyl 3-hydroxylase 1 antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling LEPRE1 with Purified ab154799 at 1/500 dilution (5µg/ml). Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-LEPRE1 antibody [EPR10193(B)] (ab154799) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : HepG2 lysate
      Lane 3 : A549 lysate
      Lane 4 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 83 kDa

    • Immunofluorescence analysis of HepG2 cells labeling LEPRE1 with ab154799 at 1/250 dilution.
    • Flow cytometric analysis of permeabilized A549 cells labeling LEPRE1 with ab154799 at 1/10 dilution (red) or a negative rabbit IgG (green).

    References

    ab154799 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT29)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Treatment
    shRNA against LEPRE1
    Specification
    HT29
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 03 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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