Recombinant Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10193(B)] to LEPRE1/P3H1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, Indirect ELISA
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LEPRE1/P3H1 antibody [EPR10193(B)]
See all LEPRE1/P3H1 primary antibodies -
Description
Rabbit monoclonal [EPR10193(B)] to LEPRE1/P3H1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, Indirect ELISAmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human LEPRE1/P3H1. The exact sequence is proprietary.
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Positive control
- HeLa, HepG2, A549 and Human placenta lysates; HepG2 and A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10193(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
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ICC/IF |
1/250 - 1/500.
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Indirect ELISA |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 83 kDa. |
ICC/IF
1/250 - 1/500. |
Indirect ELISA
Use at an assay dependent concentration. |
Target
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Function
Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts. -
Involvement in disease
Defects in LEPRE1 are the cause of osteogenesis imperfecta type 8 (OI8) [MIM:610915]. A connective tissue disorder characterized by disproportionate short stature, severe osteoporosis, shortening of the long bones, white sclerae, a round face and a short barrel-shaped chest. -
Sequence similarities
Belongs to the leprecan family.
Contains 1 Fe2OG dioxygenase domain.
Contains 4 TPR repeats. -
Post-translational
modificationsO-glycosylated; chondroitin sulfate. -
Cellular localization
Endoplasmic reticulum. Secreted > extracellular space > extracellular matrix. Secreted into the extracellular matrix as a chondroitin sulfate proteoglycan. - Information by UniProt
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Database links
- Entrez Gene: 64175 Human
- Omim: 610339 Human
- SwissProt: Q32P28 Human
- Unigene: 720014 Human
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Alternative names
- GROS 1 antibody
- GROS1 antibody
- Growth suppressor 1 antibody
see all
Images
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ELISA using ab154799 at varying antibody concentrations and antigen concentration at 1000 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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All lanes : Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Human placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 83 kDa -
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling LEPRE1/P3H1 with Purified ab154799 at 1/500 dilution (5µg/ml). Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
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Immunofluorescence analysis of HepG2 cells labeling LEPRE1/P3H1 with ab154799 at 1/250 dilution.
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Intracellular flow cytometric analysis of permeabilized A549 cells labeling LEPRE1/P3H1 with ab154799 at 1/10 dilution (red) or a negative rabbit IgG (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154799 has been referenced in 1 publication.
- Wu J et al. Characterization of PPIB interaction in the P3H1 ternary complex and implications for its pathological mutations. Cell Mol Life Sci N/A:N/A (2019). PubMed: 30993352