The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA (suggested working dilution 1:2,400) and immunoblotting (suggested working dilution 1:200 to 1:400). Reactivity in other immunoassays is unknown. Optimal titers for other applications should be determined by the researcher.
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.