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Synthetic peptide corresponding to Mouse Leptin aa 25-44.
Our Abpromise guarantee covers the use of ab3583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|Inhibition Assay||Use at an assay dependent concentration.|
|ICC||1/50 - 1/200.|
|IHC-Fr||1/50 - 1/200.|
|WB||1/200 - 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).|
Immunohistochemical staining of Leptin in Sheep brain using ab3583.
ab3583 at a dilution of 1 / 4000 staining leptin in mouse 3T3-L1 cell lysate by Western blot.