Our Abpromise guarantee covers the use of ab9749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mouse Leptin (using 100 µl/well antibody solution).
WB Use a concentration of 0.1 - 0.2 µg/ml.
IHC-P Use a concentration of 0.2 µg/ml.


  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ94114 antibody
    • LEP antibody
    • LEP_HUMAN antibody
    • LEPD antibody
    • Leptin (murine obesity homolog) antibody
    • Leptin (obesity homolog, mouse) antibody
    • Leptin antibody
    • Leptin Murine Obesity Homolog antibody
    • Leptin Precursor Obesity Factor antibody
    • OB antibody
    • Obese protein antibody
    • Obese, mouse, homolog of antibody
    • Obesity antibody
    • Obesity factor antibody
    • Obesity homolog mouse antibody
    • Obesity Murine Homolog Leptin antibody
    • OBS antibody
    • OTTHUMP00000212285 antibody
    see all

Anti-Leptin antibody images

  • ab9749 staining Leptin in murine cecum tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in 100mM citric acid. The primary antibody was used at 0.20 ug/ml and incubated with sample at 4°C overnight. A cyanine-3 conjugated donkey polyclonal to rabbit was used as secondary.

References for Anti-Leptin antibody (ab9749)

This product has been referenced in:
  • de Oliveira M  et al. Triiodothyronine Increases mRNA and Protein Leptin Levels in Short Time in 3T3-L1 Adipocytes by PI3K Pathway Activation. PLoS One 8:e74856 (2013). Read more (PubMed: 24058635) »

See 1 Publication for this product

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According to SwissProt, O-octanoylation or O-decanoylation is essential for Ghrelin activity. As this antibody detects both the octanoylated and non-octanoylated forms of Ghrelin, the antibody detects therefore both th...

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