Overview

  • Product nameAnti-Leptin antibody
    See all Leptin primary antibodies
  • Description
    Rabbit polyclonal to Leptin
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Goat, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MHWGTLCGFL WLWPYLFYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS) of Human Leptin (NP_000221).

  • Positive control
    • Human fetal small intestine lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab98090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ94114 antibody
    • LEP antibody
    • LEP_HUMAN antibody
    • LEPD antibody
    • Leptin (murine obesity homolog) antibody
    • Leptin (obesity homolog, mouse) antibody
    • Leptin antibody
    • Leptin Murine Obesity Homolog antibody
    • Leptin Precursor Obesity Factor antibody
    • OB antibody
    • Obese protein antibody
    • Obese, mouse, homolog of antibody
    • Obesity antibody
    • Obesity factor antibody
    • Obesity homolog mouse antibody
    • Obesity Murine Homolog Leptin antibody
    • OBS antibody
    • OTTHUMP00000212285 antibody
    see all

Anti-Leptin antibody images

  • Anti-Leptin antibody (ab98090) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal small intestine lysate at 10 µg

    Predicted band size : 19 kDa
  • ab98090 staining Leptin in placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-Leptin antibody (ab98090)

ab98090 has not yet been referenced specifically in any publications.

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With regards to your technical questions:

1.Did you try to block the membrane in 5% BSA instead of 5% milk? Is it 1 hour incubation at RT?
We only use 5% milk for blocking the membrane. Either...

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