Overview

  • Product nameLeptin Human ELISA Kit
    See all Leptin kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    1.64 pg/ml - 400 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.42 84% - 104%
    Serum 94.49 83% - 103%
    Plasma 93.85 82% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Leptin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Leptin in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • FLJ94114
    • LEP
    • LEP_HUMAN
    • Leptin
    • Leptin Murine Obesity Homolog
    • Leptin Precursor Obesity Factor
    • OB
    • Obese protein
    • Obesity factor
    • Obesity homolog mouse
    • Obesity murine homolog leptin
    • OBS
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Leptin Human ELISA Kit images

  • Leptin measured in cell lysates showing quantity (pg) per mg of protein

  • Leptin measured in biological fluids showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab100581.

  • Representative Standard Curve using ab100581.

Protocols

References for Leptin Human ELISA Kit (ab100581)

ab100581 has not yet been referenced specifically in any publications.

Product Wall

These products are very similar, they use similar techniques to measure human Leptin in samples. In reviewing the information about each kit I have noted that ab100581 Sensitivity< 2 pg/ml, Range of 1.64 pg/ml - 400 pg/ml and Recovery of 94%. While ...

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Thank you for contacting us. We do not specifically have data on adolescents but can tell you that typically human urine does not have to be concentrated and can be diluted using the kit if necessary.

I hope this information is helpful to you...

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Thank you for contacting us.

The differences of the two kits you are interested in are listed below. The most important difference is that one of the Kits is for detection of the Leptin Receptor, while the other kit is only detecting the sec...

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Thank you very much for your inquiry and your interest in our kits.

Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

For the other three kits, ab100581, ab100718 and ab100773,the laborat...

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Thank you for contacting Abcam. Unfortunately, we do not have any cross reactivity data for rabbit samples on the ab100581 kit. Therefore we do not know if the kit will work with rabbit serum samples. Both the capture and detection antibody are monoclo...

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Thank you for your enquriy. I am sorry to confirm that the Leptin standard protein from kit ab100581 is not sold separately. If you have any further questions, please do not hesitate to contact us.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"