Overview

  • Product nameLeptin Mouse ELISA KitSee all Leptin kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.47 83% - 103%
    Serum 93.29 82% - 102%
    Plasma 95.38 84% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Leptin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Leptin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse Leptin coated on a 96- well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the leptin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • FLJ94114
    • LEP
    • LEP_HUMAN
    • Leptin
    • Leptin Murine Obesity Homolog
    • Leptin Precursor Obesity Factor
    • OB
    • Obese protein
    • Obesity factor
    • Obesity homolog mouse
    • Obesity murine homolog leptin
    • OBS
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Leptin Mouse ELISA Kit images

  • Representative Standard Curve using ab100718

  • Representative Standard Curve using ab100718

Protocols

References for Leptin Mouse ELISA Kit (ab100718)

ab100718 has not yet been referenced specifically in any publications.

Product Wall

We have not yet tested ab100718 with cell lysates. However, the kit is compatible with many different samples types so lysates will most likely work. If you decide to test this kit with lysate samples, we recommend diluting the samples at least 5-fold ...

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Thank you very much for your inquiry and your interest in our kits.

Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

For the other three kits, ab100581, ab100718 and ab100773,the laborat...

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Thank you for your enquiry.

I have now received further information regarding the reading of the results from this kit. The manuals advise using a log-log model as this type of plot will fit most data sets very well. However, you can use any...

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Thank you for your inquiry.  This kit ab100718 has been validated for serum, plasma, and cell culture supernatant samples. Are you using whole blood samples?  If so, this kit may work, but we would not recommend it as the chances of experiencing hi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"