• Product nameAnti-Leptin Receptor antibody
    See all Leptin Receptor primary antibodies
  • Description
    Rabbit polyclonal to Leptin Receptor
  • SpecificityWill detect all isoforms of the leptin receptor.
  • Tested applicationsSuitable for: IP, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat Leptin Receptor aa 577-594 (extracellular).


    (Peptide available as ab5837)

  • Positive control
    • Mouse lung and brain extract, 293 whole cell lysate, Huvec cell lysate.
  • General notes

    As per Uniprot database (P48357) OB receptor has 5 isoforms and is highly glycosylated, this means the observed band size will be different than predicted and multiple bands are expected depending upon the isoforms expressed in sample types.



Our Abpromise guarantee covers the use of ab5593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 21151569
IHC-Fr Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 100, 125 kDa.Can be blocked with Leptin Receptor peptide (ab5837). Corresponding to the long and short forms (respectively) of leptin receptor OB-R from mouse brain extract. Two smaller bands of approximately 31 and 35 kDa are also detected, which cannot be competed out with immunogenic peptide. Block with 5% milk in TBST at RT. The native leptin receptor is a homodimer held together with disulfide bonds. On a reducing SDS-PAGE, bands may appear as approximately 60 kDa subunits. Further variations in size is possible and is due to glycosylation.


  • FunctionReceptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
  • Tissue specificityIsoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 4 fibronectin type-III domains.
    Contains 1 Ig-like (immunoglobulin-like) domain.
  • DomainThe cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 295 antibody
    • CD295 antibody
    • CD295 antigen antibody
    • Db antibody
    • Fa antibody
    • HuB219 antibody
    • LEP R antibody
    • LEP-R antibody
    • LEPR antibody
    • LEPR_HUMAN antibody
    • LEPRD antibody
    • Leptin receptor antibody
    • Leptin receptor fatty antibody
    • Leptin receptor gene related protein antibody
    • Leptin receptor precursor antibody
    • Leptin receptor precursor antibody
    • OB R gene related protein antibody
    • OB receptor antibody
    • OB-R antibody
    • OB-RGRP antibody
    • obl antibody
    • Obr antibody
    see all

Anti-Leptin Receptor antibody images

  • All lanes : Anti-Leptin Receptor antibody (ab5593) at 1/2000 dilution

    Lane 1 : Mouse lung whole cell lysate
    Lane 2 : Mouse brain whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Polyclonal goat anti-rabbit conjugated to HRP at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Additional bands at : 100 kDa (possible isoform).

    Exposure time : 10 seconds

    This image is courtesy of an anonymous abreview.

    Blocking agent: 5% Milk for 1 hour at 23°C.

    See Abreview

  • Western blot of both short and long forms of Leptin Receptor on mouse lung tissue extract using ab5593.

References for Anti-Leptin Receptor antibody (ab5593)

This product has been referenced in:
  • Trujillo ML  et al. Hyperphagia and Central Mechanisms for Leptin Resistance during Pregnancy. Endocrinology 152:1355-65 (2011). WB ; Rat . Read more (PubMed: 21303957) »
  • Garonna E  et al. Vascular endothelial growth factor receptor-2 couples cyclo-oxygenase-2 with pro-angiogenic actions of leptin on human endothelial cells. PLoS One 6:e18823 (2011). WB ; Human . Read more (PubMed: 21533119) »

See all 6 Publications for this product

Product Wall

Thank you very much for keeping me updated about the situation, and I'm sorry to hear that the new lot is also not giving the expected results.

As mentioned before, your protocol looks very similar to the protocol used by the lab to test the ...

Read More
Application Western blot
Sample Mouse Tissue lysate - whole (mouse lung, brain)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification mouse lung, brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 11 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Mouse Cell lysate - other (C2C12)
Specification C2C12
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 27 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary Hypothalamic Neurons)
Specification Primary Hypothalamic Neurons
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% NP40 in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Dr. Brian Lam

Verified customer

Submitted Feb 21 2013

Application Western blot
Sample Mouse Tissue lysate - whole (hypothalamus)
Loading amount 25 µg
Specification hypothalamus
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mrs. Judith Kranz

Verified customer

Submitted Feb 19 2013

Thank you very much for participating in our recent survey.

I am very sorry that the long form of the OBR protein is still not being picked up with this antibody. Have you tried the membrane fractionation to enrich your samples, or the Poncea...

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Thank you very much for your reply.

I'm sending a free of charge vial from a new lot on the order ***, which should arrive tomorrow.

Please keep me updated about the results with this new vial, and if there is anything else that we ...

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Thank you very much for your reply, and I apologize for the trouble with this antibody.

I look forward to hearing from you once you have discussed the options with your supervisor. Please let me know if there is anything else that we can do f...

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Thank you very much for your emails and for your patience.

I have been discussing the situation with my colleagues in the lab, and I've found out that the mouse lung lysate used for the WB on our datasheet also came from a healthy, normal-fed...

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Thank you very much for contacting us and letting us know about the trouble with this antibody.

To better understand the situation, could you confirm the following information:

1) Are the brain, lung, liver, and muscle lysates also ...

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