- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Leptin Receptor antibody
See all Leptin Receptor primary antibodies -
DescriptionRabbit polyclonal to Leptin Receptor
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SpecificityRecombinant and natural mouse OB receptor.
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse
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Immunogen
Recombinant fragment, corresponding to amino acids 217-376 of Mouse Leptin Receptor. Expressed in E. coli
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab7212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Application notes
WB: 1/2000. Predicted molecular weight: 120-140 kDa. The native leptin receptor is a homodimer held together with disulfide bonds. On a reducing SDS-PAGE, bands may appear as approximately 60 kDa subunits. Further variations in size is possible and is due to glycosylation.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionReceptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
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Tissue specificityIsoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
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Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like (immunoglobulin-like) domain. -
DomainThe cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsOn ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 16847 Mouse
- SwissProt: P48356 Mouse
- Unigene: 259282 Mouse
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Alternative names
- CD 295 antibody
- CD295 antibody
- CD295 antigen antibody
see all
Protocols
References for Anti-Leptin Receptor antibody (ab7212)
ab7212 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"