Overview

  • Product nameAnti-Leptin Receptor antibody
    See all Leptin Receptor primary antibodies
  • Description
    Chicken polyclonal to Leptin Receptor
  • Tested applicationsSuitable for: IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Recombinant Rat Leptin Receptor

Applications

Our Abpromise guarantee covers the use of ab92615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-FoFr: 1/100 - 1/600.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
    • Tissue specificityIsoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
    • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
      Contains 4 fibronectin type-III domains.
      Contains 1 Ig-like (immunoglobulin-like) domain.
    • DomainThe cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
    • Post-translational
      modifications
      On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 295 antibody
      • CD295 antibody
      • CD295 antigen antibody
      • Db antibody
      • Fa antibody
      • HuB219 antibody
      • LEP R antibody
      • LEP-R antibody
      • LEPR antibody
      • LEPR_HUMAN antibody
      • LEPRD antibody
      • Leptin receptor antibody
      • Leptin receptor fatty antibody
      • Leptin receptor gene related protein antibody
      • Leptin receptor precursor antibody
      • Leptin receptor precursor antibody
      • OB R gene related protein antibody
      • OB receptor antibody
      • OB-R antibody
      • OB-RGRP antibody
      • obl antibody
      • Obr antibody
      see all

    Anti-Leptin Receptor antibody images

    • ab92615, at 1/200 dilution, staining Leptin Receptor in rat intestine by Immunohistochemistry. Detection was done using anti-chicken Cy2-conjugated antibodies (green color). Cell nuclei were counterstained with DAPI (blue color).
    • ab92615, at 1/50 dilution, staining Leptin Receptor in 40 µm free-floating rat brainstem fixed with 4% paraformaldehyde by Immunohistochemistry, using a Rhodamine Red-X, donkey anti-chicken secondary antibody.
      Courtesy of Montina Van Meter (Drs. Rogers & Hermann - Autonomic Neuroscience Lab, Pennington Biomedical Research Center, Baton Rouge, LA)

    References for Anti-Leptin Receptor antibody (ab92615)

    ab92615 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"