Anti-Leptin Receptor antibody [LPR-01] (ab2139)


  • Product name
    Anti-Leptin Receptor antibody [LPR-01]
    See all Leptin Receptor primary antibodies
  • Description
    Mouse monoclonal [LPR-01] to Leptin Receptor
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Fusion protein, corresponding to amino acids 1-839 of Human Leptin Receptor fused to the Fc region of human IgG (with IIEGR added at the amino terminus and 6 histidine residues added at the carboxy terminus). Will detect all Leptin receptor isoforms

  • Positive control
    • Human Leptin Receptor/FC chimera (This antigen migrated as a 190-220 protein in SDS-PAGE).



Our Abpromise guarantee covers the use of ab2139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IHC-P: 1/10 - 1/100.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 132.4 kDa.
    The native leptin receptor is a homodimer held together with disulfide bonds. On a reducing SDS-PAGE, bands may appear as approximately 60 kDa subunits. Further variations in size is possible and is due to glycosylation.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
    • Tissue specificity
      Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
    • Sequence similarities
      Belongs to the type I cytokine receptor family. Type 2 subfamily.
      Contains 4 fibronectin type-III domains.
      Contains 1 Ig-like (immunoglobulin-like) domain.
    • Domain
      The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
    • Post-translational
      On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 295 antibody
      • CD295 antibody
      • CD295 antigen antibody
      • Db antibody
      • Fa antibody
      • HuB219 antibody
      • LEP R antibody
      • LEP-R antibody
      • LEPR antibody
      • LEPR_HUMAN antibody
      • LEPRD antibody
      • Leptin receptor antibody
      • Leptin receptor fatty antibody
      • Leptin receptor gene related protein antibody
      • Leptin receptor precursor antibody
      • Leptin receptor precursor antibody
      • OB R gene related protein antibody
      • OB receptor antibody
      • OB-R antibody
      • OB-RGRP antibody
      • obl antibody
      • Obr antibody
      see all

    Anti-Leptin Receptor antibody [LPR-01] images

    References for Anti-Leptin Receptor antibody [LPR-01] (ab2139)

    ab2139 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. I'm sorry there seemed to be a discrepancy between datasheets. This is because the predicted molecular weight of leptin receptor according to Swiss Prot is 132.4 kDa. The native leptin receptor is a homodimer held together ...

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    The antibody was tested in WB with the antigen LEPTIN R-AGCX made by RDI. If you have any additional questions, please contact us again.

    Thank you for your enquiry, I am unsure whether one of my colleagues has already answered your enquiry and apologise if this is the case and I repeat what you have previously been told. The predicted KDa of protein targets cited on our data sheets i...

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