Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export.
Biological descriptionPotent inhibitor of CRM-1-mediated (exportin-1) nuclear export. Displays antibiotic, antifungal, and antitumor properties and blocks the cell cycle at G1 and G2 phases. Potent inhibitor of nucleo-cytoplasmic translocation of Rev. Also shown to suppress HIV-1 replication in human monocytes (IC50 = 600 pM).
Supplied in ethanol (25 mg/ml)
Chemical name(2E,5S,6R,7S,9R,10E,12E,15R,16Z,18E)-19-[(2S,3S)-3,6-Dihydro-3-methyl-6-oxo-2H-pyran-2-yl]-17-ethyl-6-hydroxy-3,5,7,9,11,15-hexamethyl-8-oxo-2,10,12,16,18-nonadecapentaenoic acid
Storage instructionsStore at -20°C (desiccating conditions).
Solubility overviewSupplied in ethanol (25 μg/ml)
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Leptomycin B (ethanol solution) images
Functional Studies-Leptomycin B (ethanol solution)(ab120501)
ab38335 staining p75 in HeLa cells treated with Leptomycin B (ethanol solution) (ab120501), by ICC/IF. Increase in p75 expression in the nucleous and cytoplasm correlates with increased concentration of Leptomycin B (ethanol solution), as described in literature. The cells were incubated at 37°C for 2h in media containing different concentrations of ab120501 (Leptomycin B (ethanol solution)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab38335 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
References for Leptomycin B (ethanol solution) (ab120501)
Kudo N et al. Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region. Proc Natl Acad Sci U S A96:9112-7 (1999).
Read more (PubMed: 10430904) »
Wolff B et al. Leptomycin B is an inhibitor of nuclear export: inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA. Chem Biol4:139-47 (1997).
Read more (PubMed: 9190288) »