Overview

  • Product nameAnti-LETM1 antibody
    See all LETM1 primary antibodies
  • Description
    Mouse monoclonal to LETM1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 601-709 of Human LETM1

Properties

Applications

Our Abpromise guarantee covers the use of ab55434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 83 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCrucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization.
  • Sequence similaritiesContains 1 EF-hand domain.
    Contains 1 LETM1 domain.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LETM 1 antibody
    • LETM1 and EF hand domain containing protein 1, mitochondrial antibody
    • LETM1 and EF-hand domain-containing protein 1 antibody
    • Letm1 antibody
    • LETM1_HUMAN antibody
    • Leucine zipper EF hand containing transmembrane protein 1 antibody
    • Leucine zipper-EF-hand-containing transmembrane protein 1 antibody
    • Mdm38 homolog antibody
    • mitochondrial antibody
    see all

Anti-LETM1 antibody images

  • Overlay histogram showing HeLa cells stained with ab55434 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55434, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • LETM1 antibody (ab55434) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.


  • Predicted band size : 83 kDa
    LETM1 antibody (ab55434) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55434 at 10 ug/ml staining LETM1 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-LETM1 antibody (ab55434)

ab55434 has not yet been referenced specifically in any publications.

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