The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 83 kDa.
Use a concentration of 3 µg/ml.
Use a concentration of 10 µg/ml.
Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization.
LETM1 and EF hand domain containing protein 1, mitochondrial antibody
LETM1 and EF-hand domain-containing protein 1 antibody
Leucine zipper EF hand containing transmembrane protein 1 antibody
Leucine zipper-EF-hand-containing transmembrane protein 1 antibody
Mdm38 homolog antibody
Anti-LETM1 antibody images
Flow Cytometry - Anti-LETM1 antibody (ab55434)
Overlay histogram showing HeLa cells stained with ab55434 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55434, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
IHC-P - LETM1 antibody (ab55434)
LETM1 antibody (ab55434) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
Western blot - LETM1 antibody (ab55434)
Predicted band size : 83 kDa LETM1 antibody (ab55434) at 1ug/lane + HeLa cell lysate at 25ug/lane.