Overview

  • Product name
  • Description
    Mouse monoclonal to LETM1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 601-709 of Human LETM1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab55434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 83 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization.
  • Sequence similarities
    Contains 1 EF-hand domain.
    Contains 1 LETM1 domain.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LETM 1 antibody
    • LETM1 and EF hand domain containing protein 1, mitochondrial antibody
    • LETM1 and EF-hand domain-containing protein 1 antibody
    • Letm1 antibody
    • LETM1_HUMAN antibody
    • Leucine zipper EF hand containing transmembrane protein 1 antibody
    • Leucine zipper-EF-hand-containing transmembrane protein 1 antibody
    • Mdm38 homolog antibody
    • mitochondrial antibody
    see all

Anti-LETM1 antibody images

  • Overlay histogram showing HeLa cells stained with ab55434 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55434, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • LETM1 antibody (ab55434) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.


  • Predicted band size : 83 kDa
    LETM1 antibody (ab55434) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55434 at 10 ug/ml staining LETM1 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-LETM1 antibody (ab55434)

This product has been referenced in:
  • Jiang D  et al. Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome. Proc Natl Acad Sci U S A 110:E2249-54 (2013). Read more (PubMed: 23716663) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab55434.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up