Anti-LETM2 antibody - N-terminal (ab170773)

Overview

  • Product name
    Anti-LETM2 antibody - N-terminal
  • Description
    Rabbit polyclonal to LETM2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LETM2 aa 108-137 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q2VYF4

  • Positive control
    • NCI-H460 cells and lysate; Human placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab170773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 56 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Contains 1 LETM1 domain.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LETM1 and EF hand domain containing protein 2 antibody
    • LETM1 and EF-hand domain-containing protein 2 antibody
    • LETM1 domain containing protein LETM2 mitochondrial antibody
    • LETM1 domain-containing protein LETM2 antibody
    • Letm2 antibody
    • LETM2_HUMAN antibody
    • Leucine zipper EF hand containing transmembrane protein 1 like antibody
    • Leucine zipper EF hand containing transmembrane protein 2 antibody
    • Leucine zipper-EF-hand-containing transmembrane protein 1-like antibody
    • mitochondrial antibody
    see all

Images

  • Anti-LETM2 antibody - N-terminal (ab170773) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size: 56 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling LETM2 with ab170773 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of NCI-H460 cells labeling LETM2 with ab170773 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab170773 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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