Overview

  • Product nameAnti-Leucyl tRNA synthetase antibody
    See all Leucyl tRNA synthetase primary antibodies
  • Description
    Rabbit polyclonal to Leucyl tRNA synthetase
  • SpecificityThis antibody reacts specifically with Leucyl tRNA synthetase.
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    L930-H1176 denatured human recombinant LRS.

Properties

Applications

Our Abpromise guarantee covers the use of ab31534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 135 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/200.

Target

  • FunctionCatalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.
  • Involvement in diseaseInfantile liver failure syndrome 1
  • Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic antibody
    • Cytoplasmic leucyl tRNA synthetase antibody
    • Cytosolic leucyl tRNA synthetase antibody
    • FLJ10595 antibody
    • FLJ21788 antibody
    • hr025Cl antibody
    • HSPC 192 antibody
    • HSPC192 antibody
    • KIAA1352 antibody
    • LARS 1 antibody
    • Lars antibody
    • LARS1 antibody
    • Leucine translase antibody
    • Leucine tRNA ligase antibody
    • Leucine--tRNA ligase antibody
    • Leucyl tRNA synthetase cytoplasmic antibody
    • Leucyl-tRNA synthetase antibody
    • LeuRS antibody
    • LEUS antibody
    • LRS antibody
    • PIG 44 antibody
    • PIG44 antibody
    • Proliferation inducing gene 44 antibody
    • RNTLS antibody
    • SYLC_HUMAN antibody
    see all

Anti-Leucyl tRNA synthetase antibody images

  • All lanes : Anti-Leucyl tRNA synthetase antibody (ab31534) at 1/4000 dilution

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 135 kDa
    Observed band size : 135 kDa


  • Predicted band size : 135 kDa
    ab31534 used in Western Blot at a 1/4,000 dilution.
  • ab31534 at a 1/200 dilution staining Leucyl tRNA synthetase in human colon cancer tissue by Immunohistochemistry.

References for Anti-Leucyl tRNA synthetase antibody (ab31534)

This product has been referenced in:
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »
  • Guan BJ  et al. Translational control during endoplasmic reticulum stress beyond phosphorylation of the translation initiation factor eIF2a. J Biol Chem 289:12593-611 (2014). WB ; Mouse . Read more (PubMed: 24648524) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"