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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer Preservative: 0.05% Sodium Azide.
Constituents: Whole serum.
Purity Whole antiserum
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 135 kDa.
Use at an assay dependent concentration.
Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.
Involvement in disease Infantile liver failure syndrome 1
Sequence similarities Belongs to the class-I aminoacyl-tRNA synthetase family.
Cellular localization Cytoplasm.
Information by UniProt
Cytoplasmic leucyl tRNA synthetase antibody
Cytosolic leucyl tRNA synthetase antibody
Anti-Leucyl tRNA synthetase antibody images
Western blot - Leucyl tRNA synthetase antibody (ab31534)
All lanes : Anti-Leucyl tRNA synthetase antibody (ab31534) at 1/4000 dilution Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Predicted band size : 135 kDa Observed band size : 135 kDa
Western blot - Anti-Leucyl tRNA synthetase antibody (ab31534)
Predicted band size : 135 kDa ab31534 used in Western Blot at a 1/4,000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leucyl tRNA synthetase antibody (ab31534)
ab31534 at a 1/200 dilution staining Leucyl tRNA synthetase in human colon cancer tissue by Immunohistochemistry.
References for Anti-Leucyl tRNA synthetase antibody (ab31534)
This product has been referenced in:
et al. Translational control during endoplasmic reticulum stress beyond phosphorylation of the translation initiation factor eIF2a. J Biol Chem 289:12593-611 (2014).
Read more (PubMed: 24648524) »
et al. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A 110:2557-62 (2013).
Read more (PubMed: 23359686) »
See all 3 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"