The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 135 kDa.
Use at an assay dependent concentration.
FunctionCatalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.
Involvement in diseaseInfantile liver failure syndrome 1
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
ab31534 at a 1/200 dilution staining Leucyl tRNA synthetase in human colon cancer tissue by Immunohistochemistry.
References for Anti-Leucyl tRNA synthetase antibody (ab31534)
This product has been referenced in:
Park SJ et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One10:e0142253 (2015).
Read more (PubMed: 26544075) »
Guan BJ et al. Translational control during endoplasmic reticulum stress beyond phosphorylation of the translation initiation factor eIF2a. J Biol Chem289:12593-611 (2014).
Read more (PubMed: 24648524) »