Anti-Leukotriene A4 hydrolase antibody (ab23677)

Abreviews (1)Q&A (1)Specific References (1)

Overview

  • Product name
    Anti-Leukotriene A4 hydrolase antibody
    See all Leukotriene A4 hydrolase primary antibodies
  • Description
    Rabbit polyclonal to Leukotriene A4 hydrolase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Rat, Guinea pig
  • Immunogen

    Recombinant full length protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab23677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 69 kDa.

Target

Images

  • Western blot - Anti-Leukotriene A4 hydrolase antibody (ab23677)
    Western blot - Anti-Leukotriene A4 hydrolase antibody (ab23677)
    All lanes : Anti-Leukotriene A4 hydrolase antibody (ab23677) at 2 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

References

This product has been referenced in:
  • Korzekwa AJ  et al. Characterization of bovine immortalized luteal endothelial cells: action of cytokines on production and content of arachidonic acid metabolites. Reprod Biol Endocrinol 9:27 (2011). WB ; Cow . Read more (PubMed: 21349168) »

See 1 Publication for this product

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Western blot abreview for Anti-Leukotriene A4 hydrolase antibody

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Peripheral blood mononuclear cells)
Loading amount
20 µg
Specification
Peripheral blood mononuclear cells
Gel Running Conditions
Non-reduced Denaturing (12% Tris-HEPES-SDS)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

Submitted Mar 31 2009

What do you recommend as positive and negative controls for these antibodies?

Thank you for contacting us. For ab23677, LTA4 hydrolase, I suggest either a neutrophil or monocyte lysate. We carry THP1 lysate, ab7913, which is a human monocytic leukemia. For a negative control, LTA4 was detected in epidermal keratinocytes and ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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