Anti-Leukotriene B4 Receptor antibody (ab82851)

Overview

  • Product name
    Anti-Leukotriene B4 Receptor antibody
    See all Leukotriene B4 Receptor primary antibodies
  • Description
    Rabbit polyclonal to Leukotriene B4 Receptor
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Leukotriene B4 Receptor.

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Heart; Thyroid; Thymus.

Properties

Applications

Our Abpromise guarantee covers the use of ab82851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 38 kDa).

Target

  • Function
    Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.
  • Tissue specificity
    Expressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    Phosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BLT antibody
    • BLT1 antibody
    • BLTR antibody
    • Chemoattractant receptor-like 1 antibody
    • Chemokine receptor like 1 antibody
    • CMKRL1 antibody
    • G protein coupled receptor 16 antibody
    • G-protein coupled receptor 16 antibody
    • GPR16 antibody
    • Leukotriene B4 G Protein coupled receptor antibody
    • Leukotriene B4 receptor 1 antibody
    • Leukotriene B4 receptor antibody
    • LT4R1_HUMAN antibody
    • LTB4 R 1 antibody
    • LTB4-R 1 antibody
    • LTB4-R1 antibody
    • LTB4R 1 antibody
    • Ltb4r antibody
    • LTB4R1 antibody
    • LTBR1 antibody
    • P2RY7 antibody
    • P2Y purinoceptor 7 antibody
    • P2Y7 antibody
    • Purinergic receptor P2Y G protein coupled 7 antibody
    see all

Images

  • All lanes : Anti-Leukotriene B4 Receptor antibody (ab82851) at 1 µg/ml

    Lane 1 : Human thyroid normal tissue lysate - total protein (ab29636)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,62 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab82851 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82851, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.

References

ab82851 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for submitting your review for ab82851 for western blotting of rat samples. I have compared the immunogen sequence use to produce ab82851 with the that of the rat sequence. Unfortunately they are quite divergent so I would not expect this ant...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (rat ischemic liver)
Loading amount
10 µg
Specification
rat ischemic liver
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Jung nam hee

Verified customer

Submitted Feb 15 2012

I am very pleased to hear you would like to test ab82851 in mouse or rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse or rat and include this code in the “Additio...

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