Anti-Leukotriene B4 Receptor antibody (ab82851)


  • Product nameAnti-Leukotriene B4 Receptor antibody
    See all Leukotriene B4 Receptor primary antibodies
  • Description
    Rabbit polyclonal to Leukotriene B4 Receptor
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Leukotriene B4 Receptor.

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Heart; Thyroid; Thymus.



Our Abpromise guarantee covers the use of ab82851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 38 kDa).


  • FunctionReceptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.
  • Tissue specificityExpressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Post-translational
    Phosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BLT antibody
    • BLT1 antibody
    • BLTR antibody
    • Chemoattractant receptor-like 1 antibody
    • Chemokine receptor like 1 antibody
    • CMKRL1 antibody
    • G protein coupled receptor 16 antibody
    • G-protein coupled receptor 16 antibody
    • GPR16 antibody
    • Leukotriene B4 G Protein coupled receptor antibody
    • Leukotriene B4 receptor 1 antibody
    • Leukotriene B4 receptor antibody
    • LT4R1_HUMAN antibody
    • LTB4 R 1 antibody
    • LTB4-R 1 antibody
    • LTB4-R1 antibody
    • LTB4R 1 antibody
    • Ltb4r antibody
    • LTB4R1 antibody
    • LTBR1 antibody
    • P2RY7 antibody
    • P2Y purinoceptor 7 antibody
    • P2Y7 antibody
    • Purinergic receptor P2Y G protein coupled 7 antibody
    see all

Anti-Leukotriene B4 Receptor antibody images

  • All lanes : Anti-Leukotriene B4 Receptor antibody (ab82851) at 1 µg/ml

    Lane 1 : Human thyroid normal tissue lysate - total protein (ab29636)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 20 µg per lane.

    Lanes 1 - 2 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,62 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab82851 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82851, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.

References for Anti-Leukotriene B4 Receptor antibody (ab82851)

ab82851 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting your review for ab82851 for western blotting of rat samples. I have compared the immunogen sequence use to produce ab82851 with the that of the rat sequence. Unfortunately they are quite divergent so I would not expect this ant...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (rat ischemic liver)
Loading amount 10 µg
Specification rat ischemic liver
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mrs. Jung nam hee

Verified customer

Submitted Feb 15 2012

I am very pleased to hear you would like to test ab82851 in mouse or rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse or rat and include this code in the “Additio...

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