Overview

  • Product nameAnti-Leupaxin antibody
    See all Leupaxin primary antibodies
  • Description
    Mouse polyclonal to Leupaxin
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Leupaxin protein (Human)

  • Positive control
    • WB: Leupaxin transfected 293T cell lysate and human spleen tissue lysate. ICC/IF: MALME-3M (methanol fixed) cells

Properties

Applications

Our Abpromise guarantee covers the use of ab67571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • RelevanceLeupaxin is preferentially expressed in hematopoietic cells and is most homologous to the focal adhesion protein, paxillin. It may function in cell type specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by tyrosine kinase activity.
  • Cellular localizationCell Membrane, Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • LDLP antibody
    • LDPL antibody
    • leupaxin antibody

Anti-Leupaxin antibody images

  • ICC/IF image of ab67571 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab67571 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Leupaxin antibody (ab67571) at 1/500 dilution + human spleen tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • All lanes : Anti-Leupaxin antibody (ab67571) at 1/500 dilution

    Lane 1 : Leupaxin transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

References for Anti-Leupaxin antibody (ab67571)

ab67571 has not yet been referenced specifically in any publications.

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I can confirm the antibody has not been tested in mouse. The alignment of the immunogen for ab67571 shows 85% similarity to mouse protein. Due to the high alignment it is very likely to det...

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