Anti-LexA DNA Binding Region antibody (ab50953)
Key features and details
- Rabbit polyclonal to LexA DNA Binding Region
- Suitable for: WB, IP
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-LexA DNA Binding Region antibody
See all LexA DNA Binding Region primary antibodies -
Description
Rabbit polyclonal to LexA DNA Binding Region -
Host species
Rabbit -
Specificity
This antibody recognizes E. coli LexA and LexA DBD-tagged fusion proteins. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant full length protein corresponding to Escherichia coli LexA DNA Binding Region.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.035% Sodium azide
Constituents: Whole serum, 30% Glycerol -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). 1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). This product has been tested by Western blotting using a LexA-Rb fusion and a LexA-PP2A, beta subunit fusion expressed in budding yeast. The observed molecular weight of a fusion protein will be increased by approximately 24 kDa due to the addition of the LexA DNA binding domain.
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IP |
Use at 1-0.2 µg/mg of lysate.
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Notes |
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WB
1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). 1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). This product has been tested by Western blotting using a LexA-Rb fusion and a LexA-PP2A, beta subunit fusion expressed in budding yeast. The observed molecular weight of a fusion protein will be increased by approximately 24 kDa due to the addition of the LexA DNA binding domain. |
IP
Use at 1-0.2 µg/mg of lysate. |
Target
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Relevance
The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 1039346 Escherichia coli
- Entrez Gene: 948544 Escherichia coli
- Entrez Gene: 960080 Escherichia coli
- SwissProt: P0A7C2 Escherichia coli
- SwissProt: P0A7C3 Escherichia coli
- SwissProt: P0A7C4 Escherichia coli
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Alternative names
- ExrA antibody
- Lex A antibody
- LexA antibody
see all
Images
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All lanes : Anti-LexA DNA Binding Region antibody (ab50953) at 1/100000 dilution
Lane 1 : Crude yeast protein extract without a LexA fusion protein
Lane 2 : Crude yeast protein extract expressing a LexA-Retinoblastoma (Rb) fusion protein
Lysates/proteins at 20 µg per lane.
Predicted band size: 49 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab50953 has been referenced in 6 publications.
- Yap WS et al. The yeast FIT2 homologs are necessary to maintain cellular proteostasis and membrane lipid homeostasis. J Cell Sci 133:N/A (2020). PubMed: 33033181
- Bunnell BE et al. Zinc blocks SOS-induced antibiotic resistance via inhibition of RecA in Escherichia coli. PLoS One 12:e0178303 (2017). WB . PubMed: 28542496
- Odsbu I & Skarstad K DNA compaction in the early part of the SOS response is dependent on RecN and RecA. Microbiology 160:872-82 (2014). WB ; Escherichia coli . PubMed: 24615185
- Erental A et al. Apoptosis-like death, an extreme SOS response in Escherichia coli. MBio 5:e01426-14 (2014). WB ; Escherichia coli . PubMed: 25028428
- Reyes MI et al. Peptide aptamers that bind to geminivirus replication proteins confer a resistance phenotype to tomato yellow leaf curl virus and tomato mottle virus infection in tomato. J Virol 87:9691-706 (2013). PubMed: 23824791
- Zhang F et al. Disrupting vesicular trafficking at the endosome attenuates transcriptional activation by Gcn4. Mol Cell Biol 28:6796-818 (2008). WB . PubMed: 18794364