The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). 1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). This product has been tested by Western blotting using a LexA-Rb fusion and a LexA-PP2A, beta subunit fusion expressed in budding yeast. The observed molecular weight of a fusion protein will be increased by approximately 24 kDa due to the addition of the LexA DNA binding domain.
Use at 1-0.2 µg/mg of lysate.
The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies.
Lex A antibody
LexA repressor antibody
Western blot - Anti-LexA DNA Binding Region antibody (ab50953)
All lanes : Anti-LexA DNA Binding Region antibody (ab50953) at 1/100000 dilution
Lane 1 : Crude yeast protein extract without a LexA fusion protein Lane 2 : Crude yeast protein extract expressing a LexA-Retinoblastoma (Rb) fusion protein