Overview

  • Product nameAnti-LexA DNA Binding Region antibody
    See all LexA DNA Binding Region primary antibodies
  • Description
    Rabbit polyclonal to LexA DNA Binding Region
  • SpecificityThis antibody recognizes E. coli LexA and LexA DBD-tagged fusion proteins.
  • Tested applicationsSuitable for: WB, IPmore details
  • Immunogen

    Full length protein corresponding to the DNA binding domain (DBD, amino acids 1-222) of E. coli LexA.

Properties

Applications

Our Abpromise guarantee covers the use of ab50953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). 1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). This product has been tested by Western blotting using a LexA-Rb fusion and a LexA-PP2A, beta subunit fusion expressed in budding yeast. The observed molecular weight of a fusion protein will be increased by approximately 24 kDa due to the addition of the LexA DNA binding domain.
IP 1/1.

Target

  • RelevanceThe LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies.
  • Cellular localizationNuclear
  • Alternative names
    • ExrA antibody
    • Lex A antibody
    • LexA antibody
    • LexA repressor antibody
    • Spr antibody
    • Tsl antibody
    • UmuA antibody
    see all

Anti-LexA DNA Binding Region antibody images

  • All lanes : Anti-LexA DNA Binding Region antibody (ab50953) at 1/100000 dilution

    Lane 1 : Crude yeast protein extract without a LexA fusion protein
    Lane 2 : Crude yeast protein extract expressing a LexA-Retinoblastoma (Rb) fusion protein

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 49 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

References for Anti-LexA DNA Binding Region antibody (ab50953)

This product has been referenced in:
  • Odsbu I & Skarstad K DNA compaction in the early part of the SOS response is dependent on RecN and RecA. Microbiology 160:872-82 (2014). WB ; Escherichia coli . Read more (PubMed: 24615185) »
  • Erental A  et al. Apoptosis-like death, an extreme SOS response in Escherichia coli. MBio 5:e01426-14 (2014). WB ; Escherichia coli . Read more (PubMed: 25028428) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"