Anti-LGALS3BP antibody [OTI3G8] (ab123921)

Overview

  • Product name
    Anti-LGALS3BP antibody [OTI3G8]
    See all LGALS3BP primary antibodies
  • Description
    Mouse monoclonal [OTI3G8] to LGALS3BP
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to internal sequence amino acids 19-300 of Human LGALS3BP produced in E.coli (NP_005558).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY LGALS3BP cDNA; Human endometrium adenocarcinoma, prostate and bladder carcinoma tissues; COS7 cells transiently transfected by pCMV6-ENTRY LGALS3BP.
  • General notes

    Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

    The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same antibody clone.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab123921 is purified from Mouse ascites fluid by affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    OTI3G8
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 65 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • Function
    Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
  • Tissue specificity
    Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.
  • Sequence similarities
    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 1 SRCR domain.
  • Cellular localization
    Secreted. Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90K antibody
    • 90K MAC 2 BP antibody
    • Basement membrane autoantigen p105 antibody
    • BTBD17B antibody
    • Galectin 3 binding protein antibody
    • Galectin-3-binding protein antibody
    • L3 antigen antibody
    • Lectin galactoside binding soluble 3 binding protein antibody
    • Lectin galactoside-binding soluble 3-binding protein antibody
    • LG3BP_HUMAN antibody
    • LGALS3BP antibody
    • M2BP antibody
    • Mac 2 binding protein antibody
    • Mac 2 BP antibody
    • Mac-2 BP antibody
    • Mac-2-binding protein antibody
    • Mac2 BP antibody
    • MAC2BP antibody
    • Serum protein 90K antibody
    • TANGO10B antibody
    • Transport and golgi organization 10 homolog B antibody
    • Tumor associated antigen 90K antibody
    • Tumor-associated antigen 90K antibody
    see all

Images

  • All lanes : Anti-LGALS3BP antibody [OTI3G8] (ab123921) at 1/500 dilution

    Lane 1 : HEK293T cell lysate, transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY LGALS3BP cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 65 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205039)
  • ab123921 at 1/100 dilution staining LGALS3BP in HEK293T cells transfected with either pCMV6-ENTRY LGALS3BP (Red) or empty vector control plasmid (Blue) by flow cytometry.
  • ab123921 at 1/100 dilution staining LGALS3BP in COS7 cells transiently transfected with pCMV6-ENTRY LGALS3BP by Immunofluorescence.
  • ab123921 at 1/150 dilution staining LGALS3BP in Paraffin-embedded Human Endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab123921 at 1/150 dilution staining LGALS3BP in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • ab123921 at 1/150 dilution staining LGALS3BP in Paraffin-embedded Human Bladder carcinoma tissue by Immunohistochemistry.

References

ab123921 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing...

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I have looked through our orders for ab123921 and I cannot find any that use the PO# that you provided. I did find one order from July that could potentially be the correct order, but to confirm could you please either do...

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I am sorry about the issues you have been having with ab123921 and since the protocol tips my colleague provided did not prove to be successful, I will be happy to refund the cost of the antibody for you. To do so co...

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We don't know if TA503455 detects cell surface LGALS3BP or not using FACS as they permeabilize the cells. For your review, I have included the details of the FACS protocol below. The lab only has data using the transfe...

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The concentration of this lot is GR91861-1 is 0.85mg/ml. An appropriate isotype control would be ab91353, Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control. This product is shipped with a concentration of 1mg/ml. http://www.abcam.com/Mouse-IgG...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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