Overview

  • Product nameAnti-Lgi1 antibody
    See all Lgi1 primary antibodies
  • Description
    Rabbit polyclonal to Lgi1
  • Tested applicationsSuitable for: IHC-FoFr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat Lgi1.

    (Peptide available as ab33114.)

  • Positive control
    • Mouse Brain Whole Tissue Lysate, Mouse Brain (0 day) Whole Tissue Lysate and Rat Brain Whole Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab30868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionRegulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
  • Tissue specificityPredominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level).
  • Involvement in diseaseDefects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs.
  • Sequence similaritiesContains 7 EAR repeats.
    Contains 3 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationSecreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADLTE antibody
    • ADPAEF antibody
    • ADPEAF antibody
    • Epitempin 1 antibody
    • EPITEMPIN antibody
    • Epitempin-1 antibody
    • EPT antibody
    • ETL1 antibody
    • IB1099 antibody
    • leucine rich glioma inactivated 1 antibody
    • Leucine rich glioma-inactivated protein 1 antibody
    • Leucine-rich glioma-inactivated protein 1 antibody
    • LGI1 antibody
    • LGI1_HUMAN antibody
    • OTTHUMP00000020121 antibody
    • OTTHUMP00000020122 antibody
    see all

Anti-Lgi1 antibody images

  • Anti-Lgi1 antibody (ab30868) at 1/1000 dilution + Rat brain tissue lysate at 30 µg

    Secondary
    HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 64 kDa


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Lane 1 : Marker
    Lanes 2 - 4 : Anti-Lgi1 antibody (ab30868) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : Mouse Brain Whole Tissue Lysate at 20 µg
    Lane 3 : Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg
    Lane 4 : Rat Brain Whole Tissue Lysate at 20 µg

    Secondary
    Lanes 2 - 4 : IR Dye 680 Conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilution


    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
  • ab30868 at 10ug/ml staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized and blocked before incubation with the antibody for 16 hours. An Alexa Fluor ® 488 conjugated goat polyclonal antibody was used as the secondary.

    See Abreview

  • IHC-FoFr image of Lgi1 (ab30868) staining in rat spinal cord sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat and the immunostainings were preformed using the ‘free floating’ technique. Image B is a higher power magnification of the Image A, at the level of the interface between the white and grey matter in the lamina V. Numbers in image A are Rexed's laminae numbers.

    See Abreview

References for Anti-Lgi1 antibody (ab30868)

This product has been referenced in:
  • Yokoi N  et al. Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial epilepsy. Nat Med 21:19-26 (2015). Read more (PubMed: 25485908) »
  • Boillot M  et al. Glutamatergic neuron-targeted loss of LGI1 epilepsy gene results in seizures. Brain 137:2984-96 (2014). Mouse . Read more (PubMed: 25234641) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Rat Tissue lysate - whole (Brain)
Specification Brain
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jul 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted Sep 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Loading amount 20 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (brain)
Loading amount 25 µg
Specification brain
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

Submitted Jan 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (CHO)
Specification CHO
Fixative Paraformaldehyde
Permeabilization Yes - 0.08 % to 0.2 % triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 24 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"