The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
Use a concentration of 10 µg/ml.
Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level).
Involvement in disease
Defects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs.
Lane 1 : Marker Lanes 2 - 4 : Anti-Lgi1 antibody (ab30868) at 1 µg/ml
Lane 1 : As above Lane 2 : Mouse Brain Whole Tissue Lysate at 20 µg Lane 3 : Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg Lane 4 : Rat Brain Whole Tissue Lysate at 20 µg
Secondary Lanes 2 - 4 : IR Dye 680 Conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 64 kDa Observed band size : 64 kDa
Immunocytochemistry/ Immunofluorescence - Lgi1 antibody (ab30868)This image is courtesy of an anonymous Abreview
ab30868 at 10ug/ml staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized and blocked before incubation with the antibody for 16 hours. An Alexa Fluor ® 488 conjugated goat polyclonal antibody was used as the secondary.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Lgi1 antibody (ab30868)This image is courtesy of an abreview submitted by Sophie Pezet, ESPCI, France
IHC-FoFr image of Lgi1 (ab30868) staining in rat spinal cord sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat and the immunostainings were preformed using the ‘free floating’ technique.
Image B is a higher power magnification of the Image A, at the level of the interface between the white and grey matter in the lamina V. Numbers in image A are Rexed's laminae numbers.