• Product nameAnti-Lgi1 antibody
    See all Lgi1 primary antibodies
  • Description
    Rabbit polyclonal to Lgi1
  • Specificityab69491 is predicted to be specific to Lgi1 and not recognize other Lgi proteins.
  • Tested applicationsSuitable for: IHC-FrFl, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 13 amino acid peptide from near the amino terminus of human Lgi1.

  • Positive control
    • Mouse brain tissue lysate.


Associated products


Our Abpromise guarantee covers the use of ab69491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl Use a concentration of 2 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).


  • FunctionRegulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
  • Tissue specificityPredominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level).
  • Involvement in diseaseDefects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs.
  • Sequence similaritiesContains 7 EAR repeats.
    Contains 3 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Post-translational
  • Cellular localizationSecreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADLTE antibody
    • ADPAEF antibody
    • ADPEAF antibody
    • Epitempin 1 antibody
    • EPITEMPIN antibody
    • Epitempin-1 antibody
    • EPT antibody
    • ETL1 antibody
    • IB1099 antibody
    • leucine rich glioma inactivated 1 antibody
    • Leucine rich glioma-inactivated protein 1 antibody
    • Leucine-rich glioma-inactivated protein 1 antibody
    • LGI1 antibody
    • LGI1_HUMAN antibody
    • OTTHUMP00000020121 antibody
    • OTTHUMP00000020122 antibody
    see all

Anti-Lgi1 antibody images

  • All lanes : Anti-Lgi1 antibody (ab69491) at 1 µg/ml

    Lane 1 : mouse brain tissue lysate
    Lane 2 : mouse brain tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
    Additional bands at : 85 kDa (possible non-specific binding).
  • ab69491 staining Lgi1 in mouse brain tissue by immunohistochemistry - free floating. The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 4°C. A biotin conjugated goat anti-rabbit antibody, diluted 1/250, was used as the secondary.

    See Abreview

References for Anti-Lgi1 antibody (ab69491)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Mouse Tissue sections (brain)
Specification brain

Abcam user community

Verified customer

Submitted Oct 15 2009

Application Western blot
Sample Mouse Cell lysate - whole cell (COS)
Loading amount 20 µg
Specification COS
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 25 2008