Anti-Lgi1/EPT antibody [N283/7] (ab186296)
Key features and details
- Mouse monoclonal [N283/7] to Lgi1/EPT
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Lgi1/EPT antibody [N283/7]
See all Lgi1/EPT primary antibodies -
Description
Mouse monoclonal [N283/7] to Lgi1/EPT -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Chimpanzee, Orangutan -
Immunogen
Recombinant fragment corresponding to Mouse Lgi1/EPT aa 1-150. NCBI Accession No. NP_064674.1. Fragment corresponding to LRRNT domain and first LRR repeat.
Database link: Q9JIA1 -
Positive control
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
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General notes
The clone number has been updated from S283-7 to N283/7, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N283/7 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 64 kDa).
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 64 kDa). |
Target
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Function
Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival. -
Tissue specificity
Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level). -
Involvement in disease
Defects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs. -
Sequence similarities
Contains 7 EAR repeats.
Contains 3 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 450619 Chimpanzee
- Entrez Gene: 9211 Human
- Entrez Gene: 100172937 Orangutan
- Entrez Gene: 252892 Rat
- Omim: 604619 Human
- SwissProt: Q1EGL2 Chimpanzee
- SwissProt: O95970 Human
- SwissProt: Q5R945 Orangutan
see all -
Alternative names
- ADLTE antibody
- ADPAEF antibody
- ADPEAF antibody
see all
Images
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SK-N-BE (Human neuroblastoma cell line ) cells stained for Lgi1/EPT (Green) using ab186296 at a 1/100 dilution (1 hour at RT) in ICC/IF. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 1 hour at RT.
Cells were fixed with 4% formaldehyde for 15 minutes at RT.
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Anti-Lgi1/EPT antibody [N283/7] (ab186296) at 1/1000 dilution (for 1 hour at RT) + Rat brain lysate, for 1 hour at RT at 10 µg
Secondary
Goat Anti-Mouse HRP at 1/200 dilution
Predicted band size: 64 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab186296 has not yet been referenced specifically in any publications.