Overview

  • Product name
  • Description
    Rabbit polyclonal to Lgi4
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    14 amino acid synthetic peptide from near the center of Lgi4 (Human)

  • Positive control
    • Human Brain Cerebellum, Heart and Skeletal Muscle tissue; Human brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab115305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 59 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • ab115305, at 5µg/ml, staining Lgi4 in formalin-fixed, paraffin-embedded Human Brain, Cerebellum by Immunohistochemistry, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115305, at 5µg/ml, staining Lgi4 in formalin-fixed, paraffin-embedded Human Heart tissue by Immunohistochemistry, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115305, at 5µg/ml, staining Lgi4 in formalin-fixed, paraffin-embedded Human Skeletal Muscle tissue by Immunohistochemistry, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Lane 1 : Anti-Lgi4 antibody (ab115305) at 1 µg/ml
    Lane 2 : Anti-Lgi4 antibody (ab115305) at 2 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate


    Predicted band size : 59 kDa

References

ab115305 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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