Product is received as an ammounium sulfate precipitated antibody. To reconstitute the antibody, Centrifuge antibody vial at 5,000 rpm for 5 minutes. Remove the ammonium sulfate/PBS buffer solution and discard. It's not essential to remove all of this solution: 10 µL of residual solution will not affect the resuspension of the antibody. Don't let the protein pellet dry. Resuspend the antibody pellet in PBS/TBS (pH 7.3-7.5)to a final concentration of 1.0 mg/mL. Add the liquid buffer to the pellet (DO NOT VORTEX) Mix by gentle stirring. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use.
The rehydrated antibody solution can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.
For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/200 - 1/1000. Fix with cold Acetone/Methanol (50/50) for 2 min.
IHC-FoFr: 1/200 - 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types.