Anti-Lhx2 antibody (ab78363)

Overview

  • Product nameAnti-Lhx2 antibody
    See all Lhx2 primary antibodies
  • Description
    Rabbit polyclonal to Lhx2
  • Tested applicationsSuitable for: IHC-FoFr, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    RDQPYPSSQKTKRMRT

    , corresponding to amino acids 239-254 of Human Lhx2

  • General notesProduct is received as an ammounium sulfate precipitated antibody. To reconstitute the antibody, Centrifuge antibody vial at 5,000 rpm for 5 minutes. Remove the ammonium sulfate/PBS buffer solution and discard. It's not essential to remove all of this solution: 10 µL of residual solution will not affect the resuspension of the antibody. Don't let the protein pellet dry. Resuspend the antibody pellet in PBS/TBS (pH 7.3-7.5)to a final concentration of 1.0 mg/mL. Add the liquid buffer to the pellet (DO NOT VORTEX) Mix by gentle stirring. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. The rehydrated antibody solution can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Properties

Applications

Our Abpromise guarantee covers the use of ab78363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
IHC-Fr
  • Application notesIHC-Fr: 1/200 - 1/1000. Fix with cold Acetone/Methanol (50/50) for 2 min.
    IHC-FoFr: 1/200 - 1/1000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types.
    • Sequence similaritiesContains 1 homeobox DNA-binding domain.
      Contains 2 LIM zinc-binding domains.
    • DomainLIM domains are necessary for transcription activation.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • hLhx2 antibody
      • Homeobox protein LH-2 antibody
      • Homeobox protein LH2 antibody
      • LH2 antibody
      • LHX2 antibody
      • LHX2_HUMAN antibody
      • LIM homeobox 2 antibody
      • LIM homeobox protein 2 antibody
      • LIM HOX gene 2 antibody
      • LIM/homeobox protein Lhx2 antibody
      see all

    References for Anti-Lhx2 antibody (ab78363)

    ab78363 has not yet been referenced specifically in any publications.

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    Your credit note ID is XXXXXX.

    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
    Sample Mouse Cell (keratinocyte)
    Specification keratinocyte
    Permeabilization No
    Fixative acetone/methanol
    Username

    Dr. Yong Li

    Verified customer

    Submitted Aug 23 2013

    Thank you for confirming the details.

    It seems you haven't tried acetone fixation which is necessary in IHC-Fr. The Lhx2 target is nuclear protein and acetone does the necessary permeabilization for entry of antibody in cells. Please follow t...

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    Thank you for your enquiry regarding ab78363 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for supplying an image, as this has...

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    Thank you for contacting us. Your credit note ID is 17862. I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"